P7181
Polyethylene glycol solution
Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma
동의어(들):
PEG Solution, Polyethylene Glycol
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제품정보 (DICE 배송 시 비용 별도)
grade
Hybri-Max™
Quality Segment
sterility
0.2 μm filtered
product line
BioReagent
form
solution
mol wt
average mol wt 1,450
concentration
50 % (w/v)
technique(s)
cell culture | hybridoma: suitable
impurities
endotoxin, tested
storage temp.
2-8°C
SMILES string
C(CO)O
InChI
1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
InChI key
LYCAIKOWRPUZTN-UHFFFAOYSA-N
General description
Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.
Application
Recommended for use in a normal fusion protocol requiring 50% PEG.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Polyethylene glycol solution has been used in the hybridization of the following:
- irradiated camel fibroblast cells with A23 hamster cells
- mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells
- rabbit splenocytes and cell line 240E-1
Biochem/physiol Actions
Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.
Packaging
Packaged in sealed ampules under nitrogen.
Preparation Note
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.
Other Notes
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
212.0 °F - closed cup
flash_point_c
100 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter