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MABE1867-100UG Anti-CLOCK Antibody, clone CLSP4

Anti-CLOCK, clone CLSP4, Cat. No. MABE1867, is a mouse monoclonal antibody that detects CLOCK and is tested for use in Immunocytochemistry, Immunofluorescence. Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

MABE1867-100UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1867-100UG
      DescriptionAnti-CLOCK Antibody, clone CLSP4
      Alternate Names
      • mCLOCK
      • Circadian locomoter output cycles protein kaput
      Background InformationCircadian locomoter output cycles protein kaput (UniProt: O08785; also known as mCLOCK; EC: 2.3.1.48) is encoded by the Clock gene (Gene ID: 12753) in murine species. CLOCK acts as a transcriptional activator that forms a core component of the circadian clock. CLOCK contains 2 PAS domains (aa 107-177 Pas1 domain; aa 262-332 Pas2 domain) and has a nuclear localization signaling sequence (aa 32-47). The major components of circadian clock include CRY proteins, CLOCK or NPAS2, ARNTL/BMAL1 or ARNTL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Transcription and translation of core clock components play a critical role in rhythm generation, whereas delays imposed by post-translational modifications are important for determining the period (tau) of the rhythms. CLOCK forms a heterodimer with ARNTL/BMAL1 and this heterodimer is required for E-box-dependent transactivation and for CLOCK nuclear translocation and degradation. It is also essential for phosphorylation of both CLOCK and ARNTL/BMAL1. CLOCK has an intrinsic acetyltransferase activity, which enables circadian chromatin remodeling by acetylating histones and non-histone proteins, including ARNTL/BMAL1. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 are reported to form the positive limb of the feedback loop, and as a heterodimer activate the transcription of core clock genes and clock-controlled genes. PER1/2/3 and CRY1/2 act as transcriptional repressors and form the negative limb of the feedback loop and inhibit transcription activation. CLOCK is expressed equally in brain, eye, testes, ovaries, liver, heart, lung, and kidney. In the brain, expression is abundant in the suprachiasmatic nuclei, pyriform cortex, and in the hippocampus. (Ref.: Umemura, Y., et al. (2017). Proc. Natl Acad. Sci. USA. 114(36); E7479-E7488; Yoshitane, H., et al. (2009). Mol. Cell Biol. 29(13); 3675-3686).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ200
      Applications
      ApplicationAnti-CLOCK, clone CLSP4, Cat. No. MABE1867, is a mouse monoclonal antibody that detects CLOCK and is tested for use in Immunocytochemistry, Immunofluorescence. Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesTested Applications

      Immunofluorescence Analysis: A representative lot detected CLOCK in Immunofluorescence applications (Umemura, Y., et al. (2017). Proc Natl Acad Sci USA. 114(36):E7479-E7488).

      Western Blotting Analysis: A representative lot detected CLOCK in Western Blotting applications (Umemura, Y., et al. (2017). Proc Natl Acad Sci USA. 114(36):E7479-E7488).

      Immunoprecipitation Analysis: A representative lot detected CLOCK in Immunoprecipitation applications (Yoshitane, H., et al. (2009). Mol Cell Biol. 29(13):3675-86).

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected CLOCK in Immunohistochemistry applications (Umemura, Y., et al. (2017). Proc Natl Acad Sci USA. 114(36):E7479-E7488).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenMBP-tagged recombinant fragment corresponding to 180 amino acids from the internal region of mouse CLOCK.
      EpitopeInternal
      CloneCLSP4
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone CLSP4 is a mouse monoclonal antibody that detects CLOCK protein. It targets an epitope within the internal region.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Clock
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight96.39 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in U2OS cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CLOCK in U2OS cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABE1867-100UG 04065271058927

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