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SC1000 Anti-Nanog Rabbit pAb

This Anti-Nanog Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of Nanog.

Anti-Nanog Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Nanog Homeobox, Anti-ENK, Anti-Early Embryo Specific Expression NK Family Gene

SC1000
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      OverviewRecognizes the ~40 kDa Nanog protein in mouse myeloid and embryonic stem cells.

      This product has been discontinued.
      Catalogue NumberSC1000
      Brand Family Calbiochem®
      SynonymsAnti-Nanog Homeobox, Anti-ENK, Anti-Early Embryo Specific Expression NK Family Gene
      Application Data
      Panel A: Detection of mouse Nanog by immunoblotting. Samples: Whole cell lysate from mouse myleoid cells (15 µg, lane 1) and mouse embryonic stem cells (5 µg, lane 2). Primary antibody: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (0.1 µg/ml). Detection: chemiluminescence. Panel B: Detection of mouse Nanog by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from mouse embryonic stem cells. Antibody for IP: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (1 µg/mg total protein). Immunoblotting conditions: same as panel A.
      References
      ReferencesChambers, I., et al. 2003. Cell 113, 643.
      Mitsui, K., et al. 2003. Cell 113, 631.
      Pan, G.J. and Pei, D.Q. 2003. Cell Res. 13, 499.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlMouse myeloid embryonic stem cells
      Preservative≤0.1% sodium azide
      Quality SegmentMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:5000-1:15,000)
      Immunoprecipitation (1-2 µg/ml lysate)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids within residues 1-50 of mouse Nanog
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      SC1000 0

      Documentation

      Anti-Nanog Rabbit pAb Certificates of Analysis

      TitleLot Number
      SC1000

      References

      Reference overview
      Chambers, I., et al. 2003. Cell 113, 643.
      Mitsui, K., et al. 2003. Cell 113, 631.
      Pan, G.J. and Pei, D.Q. 2003. Cell Res. 13, 499.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-October-2007 RFH
      SynonymsAnti-Nanog Homeobox, Anti-ENK, Anti-Early Embryo Specific Expression NK Family Gene
      ApplicationImmunoblotting (1:5000-1:15,000)
      Immunoprecipitation (1-2 µg/ml lysate)
      Application Data
      Panel A: Detection of mouse Nanog by immunoblotting. Samples: Whole cell lysate from mouse myleoid cells (15 µg, lane 1) and mouse embryonic stem cells (5 µg, lane 2). Primary antibody: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (0.1 µg/ml). Detection: chemiluminescence. Panel B: Detection of mouse Nanog by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate from mouse embryonic stem cells. Antibody for IP: Anti-Nanog Rabbit pAb (Cat. No. SC1000) (1 µg/mg total protein). Immunoblotting conditions: same as panel A.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40 kDa Nanog protein.
      BackgroundNanog is a newly identified homeodomain gene product that directs propagation of undifferentiated embryonic stem (ES) cells and was named after the mythological Celtic land of the eternal youth, Tir Na Nog. Nanog is present in both mouse and human embryonic stem cells, and lacking in differentiated cells. It is restricted to founder cells from which ES cells can be derived. Nanog is important for maintaining the self-renewing properties of mouse embryonic stem cells and is expressed in the cells of the early embryo that are used to derive embryonic stem cells. Current research suggests that Nanog acts independently of another signal transduction pathway important to maintaining the pluripotency of stem cells, which involves the activation of Stat3.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids within residues 1-50 of mouse Nanog
      IsotypeIgG
      Speciesmouse
      Positive controlMouse myeloid embryonic stem cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesChambers, I., et al. 2003. Cell 113, 643.
      Mitsui, K., et al. 2003. Cell 113, 631.
      Pan, G.J. and Pei, D.Q. 2003. Cell Res. 13, 499.