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69968 Biotinylated T7•Tag® Monoclonal Antibody

Biotinylated T7•Tag® Monoclonal Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69968
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      When used with Novagen's Streptavidin Alkaline Phosphatase Conjugate, Biotinylated T7•Tag Antibody enables a 5-fold increase in detection sensitivity of target proteins on Western blots compared to an anti-IgG conjugate strategy. This form of the antibody also enables specific detection in the presence of other mouse IgGs.

      The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

      This antibody is also available as a direct conjugate with alkaline phosphatase or horseradish peroxidase.

      SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
      Species/IsotypeMouse monoclonal IgG2b
      Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
      Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
      < 1 ng (developed with chemiluminescent substrates)
      FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
      Working dilution1:5,000–1:10,000
      Catalogue Number69968
      Brand Family Novagen®
      References
      Product Information
      Components
      Quality SegmentMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      69968 0

      Documentation

      Biotinylated T7•Tag® Monoclonal Antibody Certificates of Analysis

      TitleLot Number
      69968

      Citations

      Title
    • Hiroko Hayakawa, et al. (2007) Soluble ST2 blocks interleukin-33 signaling in allergic airway inflammation. Journal of Biological Chemistry 282, 26369-26380.
    • Paul H. Bessette, Jeffrey J. Rice and Patrick S. Daugherty. (2004) Rapid isolation of high-affinity protein binding peptides using bacterial display. Protein Engineering Design and Selection 17, 731-739.
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      User Protocols

      Title
      TB106 Biotinylated T7•Tag® Monoclonal Antibody

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies