Greener Alternative Detergents for MS Sample Preparation in Proteomics

WEBINAR

Effective sample preparation is crucial for accurate protein quantification in mass spectrometry-based proteomics, especially during protein extraction. However, many widely used surfactants for cell lysis, such as NP-40 Alternative, are derived from alkylphenol ethoxylates, which can break down into environmentally harmful pollutants. This session will emphasize the importance of greener proteomics, advocating for the adoption of more sustainable reagents and practices.

We will present our evaluation of 10 biodegradable surfactants across three key protocols:

• Explore the significance of greener proteomics and improved practices in research.
• Learn how 10 alternative surfactants compare to conventional detergents in various protein extraction methods.
• Understand the impact of these detergents on protein identification and analysis of post-translational modifications.
• Discover how these greener alternatives can maintain quantitative precision and proteomic depth for more sustainable practices in mass spectrometry.

Several greener detergents demonstrated performance comparable to traditional reagents. Our findings reveal that ECO Brij^® C10, L23, O20, and Tergitol^® 15-S-7 provided protein and peptide identifications on par with Triton controls. Additionally, ECO Surf^® EH-9 demonstrated effective glycoprotein enrichment, mirroring SDS-based profiles while capturing unique proteins. For histone PTMs, ECO Brij^® C10 and L23 closely matched the modification patterns seen with NP-40 Alternative.

This webinar will showcase how greener surfactants can replace conventional detergents in mass spectrometry workflows, supporting high-quality data collection and promoting environmentally responsible practices in proteomics.