Reflecting on Chiral: Strategies and Tools for Efficient Enantiomer Separations

WEBINAR

The separation and analysis of chiral compounds by liquid chromatography has been, and continues to be, an important topic, especially in the pharmaceutical industry, for the past few decades. It is well known that the enantiomeric purity, or lack thereof, in a pharmaceutical formulation can lead to drastic changes in the performance of that drug to inhibit the progression of a disease or, at worse, can make the drug toxic to the patient. Therefore, methods to gauge the enantiomeric purity of pharmaceuticals are a clear requirement and need by analytical chemists in the pharmaceutical industry. To that end, chiral stationary phases have been developed and used to characterize these racemic mixtures.

The focus of this seminar is to provide an overview of different strategies and methods employed over the past several years in characterizing samples containing chiral compounds. Aspects of chiral method development, troubleshooting, and stationary phase screening will be demonstrated. Finally, application examples, including sample prep from complex matrices, will be used to showcase complete solutions for chiral LC and LC-MS workflows.

The engaged audience member, upon listening to the webinar, will learn:

The different modes of chiral chromatography and when to employ them
How to develop a robust chiral screening method and system
How different method variables affect chiral resolution
Aspects of troubleshooting chiral separations