HPLC Column Fundamentals: Stationary Phase Selectivity

WEBINAR

This 4-part Supelco web seminar provides an introduction to how solutes can interact with columns containing porous particles (or stationary phases) to result in a chromatography separation. The efficiency, retention and selectivity terms in the resolution equation will be explained as well as how certain particle and column characteristics can alter the terms and affect resolution. The presentation should be suitable for new HPLC users, also for those who have some experience and need a quick refresher on measurement and interpretation of column performance.

Seminar Abstract

The web seminar covers the following points:

• The separation process on a column that is filled with porous particles - how the sample is introduced and transported through the column by a mobile phase.
• The well-known Resolution Equation along with a further definition of how the terms are calculated.
• Retention factor, k (or capacity factor); Selectivity (separation factor); Column Efficiency.
• How to calculate Peak Symmetry
• Benefits and drawbacks of porous silica particles as an HPLC column platform
• Important HPLC Variables - particle size, bonding chemistry, choice of column phase selection
• C18 stationary phase; modern alkyl phases
• Polar reversed-phase columns as alternatives to C18 and C8 - embedded polar group, amide phase, phenyl phase, fluorinated aromatic phase
• Supelco HPLC columns
• Steps to developing an HPLC method
• Method development considerations with polar reversed-phase columns