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AB4235 Anti-AMACR Antibody

Detect AMACR using this Anti-AMACR Antibody validated for use in WB, IH(P).

Anti-AMACR Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Tumor Markers Gene Symbol: AMACR, RACE, EC 5.1.99.4 UniProt Number: Q9UHK6
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AB4235
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB4235
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-AMACR Antibody
      Alternate Names
      • a-methylacyl-CoA racemase
      • P504S
      Background InformationProstate-specific antigen (PSA) screening for prostate cancer is now widespread in the United States among men of all ages. However PSA has limited specificity because benign disease, including prostatic enlargement and inflammation, can increase PSA levels. Thus, a more specific prostate cancer markers is needed. One such potential marker is AMACR, an enzyme that is involved in peroxisomal beta-oxidation of dietary branched-chain fatty acids. Recent studies have shown that, compared with expression in normal or benign prostate epithelium, AMACR is consistently overexpressed in prostate cancer epithelium, making it a specific marker for cancer cells within the prostate gland. Furthermore, overexpression of AMACR may increase the risk of prostate cancer because its expression is increased in premalignant lesions (prostatic intraepithelial neoplasia).
      References
      Product Information
      FormatAffinity Purified
      PresentationPeptide affinity purified antibody. Liquid in PBS, with 0.02% sodium azide as a preservative.
      Quality SegmentMQ300
      Applications
      ApplicationDetect AMACR using this Anti-AMACR Antibody validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1:500

      Immunohistochemistry: 1:100 in an ABC method. An incubation period of 30 minutes at room temperature is suggested. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic human AMACR peptide.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes human AMACR (alpha-methylacyl-CoA racemase).
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAlpha-methylacyl-CoA racemase is responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers, which are the only stereoisomers that can be degraded via peroxisomal beta-oxidation.[supplied by OMIM]
      Gene Symbol
      • AMACR
      • RACE
      • EC 5.1.99.4
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UHK6 # Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
      SIZE: 382 amino acids; 42360 Da
      SUBCELLULAR LOCATION: Peroxisome. Mitochondrion.
      DISEASE: SwissProt: Q9UHK6 # Defects in AMACR are the cause of AMACR deficiency [MIM:604489]. It results in elevated concentrations of pristanic acid. It is associated with a form of adult onset sensory motor neuropathy. & Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4) [MIM:214950]; also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
      SIMILARITY: SwissProt: Q9UHK6 ## Belongs to the caiB/baiF CoA-transferase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8ºC in undiluted aliquots for 3 months or at -20°C for up to one year from date of receipt. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      AB4235 04053252508134

      Documentation

      Anti-AMACR Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-AMACR Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY - 2368696 2368696
      RABBIT ANTI-AMACR (P504S) - 3286444 3286444
      RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY 3063422
      RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY - 2361783 2361783
      RABBIT ANTI-AMACR (P504S) POLYCLONAL ANTIBODY - 2365780 2365780

      References

      Reference overviewPub Med ID
      P504S immunohistochemical detection in 405 prostatic specimens including 376 18-gauge needle biopsies.
      Beach, R, et al.
      Am. J. Surg. Pathol., 26: 1588-96 (2002)  2002

      		12459625 12459625

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies