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MABS1286 Anti-EPO-R Antibody, clone BCO-3H2

Anti-EPO-R, clone BCO-3H2, Cat. No. MABS1286, is a rat monoclonal antibody that detects erythropoietin receptor and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

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MABS1286
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS1286
      DescriptionAnti-EPO-R Antibody, clone BCO-3H2
      Alternate Names
      • Erythropoietin receptor
      Background InformationErythropoietin receptor (UniProt: P19235; also known as EPO-R) is encoded by the EPOR gene (Gene ID: 2057) in human. EPO-R is a single-pass type 1 membrane protein that mediates erythropoietin-induced erythroblast proliferation and differentiation. It is synthesized with a signal peptide (aa 1-24) that is cleaved to produce the mature form. Upon EPO stimulation, EPO-R homodimerizes and gets phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins. The EPO-R is expressed in erythroid cells and erythroid progenitor cells. Three isoforms of EPO-R has been described that are produced by alternate splicing. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells. Mutation in EPOR gene are linked to familial erythrocytosis that is characterized by elevated serum red blood cell mass, hemoglobin and hematocrit, and increased sensitivity of erythroid progenitors to erythropoietin.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
      Quality SegmentMQ100
      Applications
      ApplicationAnti-EPO-R, clone BCO-3H2, Cat. No. MABS1286, is a rat monoclonal antibody that detects erythropoietin receptor and has been tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A representative lot detected EPO-R in Immunohistochemistry applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

      Immunoprecipitation Analysis: A representative lot detected EPO-R in Immunoprecipitation applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

      Immunofluorescence Analysis: A representative lot detected EPO-R in Immunofluorescence applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).

      Western Blotting Analysis: A representative lot detected EPO-R in Western Blotting applications (Maxwell, P., et. al. (2015). Br J Haematol. 168(3):429-42).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the human erythropoietin receptor.
      Epitopecytoplasmic domain
      CloneBCO-3H2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone BCO-3H2 is a rat monoclonal antibody that detects erythopoietin receptor in human cells. It targets an epitope with in 17 amino acids from the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EPOR
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~58 kDa observed; 55.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in UT-7 cell lysates.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected EPO-R in 10 µg of UT-7 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABS1286 04054839316029

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