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MABE148 Anti-HMGB1 Antibody, clone HMG1-5H6

Use Anti-HMGB1 Antibody, clone HMG1-5H6 (Hamster Monoclonal Antibody) validated in WB, IF, FC to detect HMGB1 also known as High-mobility group box 1.

Anti-HMGB1 Antibody, clone HMG1-5H6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Cell Cycle, DNA Replication & Repair Gene Symbol: HMGB1, HMG1, Amphoterin, HMG3, SBP-1 UniProt Number: P09429
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MABE148
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RWB, IF, FCHtPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE148
      DescriptionAnti-HMGB1 Antibody, clone HMG1-5H6
      Alternate Names
      • High mobility group protein 1
      • Sulfoglucuronyl carbohydrate binding protein
      • high mobility group box 1
      • high-mobility group (nonhistone chromosomal) protein 1
      • high-mobility group box 1
      Background InformationHMG-1 (High-Mobility-Group protein, amphoterin) is a highly conserved protein with more than 95% amino acid identity between rodent and human HMG-1. It is a prevalent non-histone chromatin component and a non-sequence specific DNA binding protein. HMG-1 consists of two homologous HMG boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMG-1 is involved in the regulation of chromatin structure as well as being involved, either as positive or negative factors with various aspects of DNA replication, transcription, repair, and ligation. HMG-1, identified as a membrane associated protein termed “amphoterin,” mediates neurite outgrowth, tumor outgrowth, and metastasis. It participates in plasminogen activation and is recognized as a late mediator of endotoxin lethality in mice.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cell lysate
      PresentationPurified hamster monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationUse Anti-HMGB1 Antibody, clone HMG1-5H6 (Hamster Monoclonal Antibody) validated in WB, IF, FC to detect HMGB1 also known as High-mobility group box 1.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Flow Cytometry
      Application NotesImmunofluorescence Analysis: 10 µg/mL from a representative lot detected HMGB1 in NIH/3T3 cells. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)

      Flow Cytometry Analysis: A representative lot detected HMGB1 in RAW264.7 cells treated with Cytofix/Cytoperm. (Image courtesy of Dr. Hideo Yagita, Juntendo University School of Medicine.)
      Biological Information
      ImmunogenRecombinant protein corresponding to mouse HMGB1.
      CloneHMG1-5H6
      HostHamster
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HMGB1
      • HMG1
      • Amphoterin
      • HMG3
      • SBP-1
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryDNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
      Molecular Weight~ 30 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

      Western Blot Analysis: 1:500 dilution of this antibody detected HMGB1 on 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABE148 04053252415401

      Documentation

      Anti-HMGB1 Antibody, clone HMG1-5H6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-HMGB1 Antibody, clone HMG1-5H6 Certificates of Analysis

      TitleLot Number
      Anti-HMGB1, clone HMG1-5H6 2830888
      Anti-HMGB1, clone HMG1-5H6 - 2115677 2115677
      Anti-HMGB1, clone HMG1-5H6 - 2033662 2033662
      Anti-HMGB1, clone HMG1-5H6 - 2058957 2058957
      Anti-HMGB1, clone HMG1-5H6 - 2172766 2172766
      Anti-HMGB1, clone HMG1-5H6 - 2234207 2234207
      Anti-HMGB1, clone HMG1-5H6 - 3241194 3241194
      Anti-HMGB1, clone HMG1-5H6 - 3767431 3767431
      Anti-HMGB1, clone HMG1-5H6 - 3902120 3902120
      Anti-HMGB1, clone HMG1-5H6 - 3903738 3903738
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