324725
Endoglycosidase F1
Cleaves specific N-glycan structures, suitable for Mass Spectrometry, from Elizabethkingia meningosepticum, recombinant, expressed in E. coli
동의어(들):
Endo-β-N-acetylglucosaminidase F1, Endo F1
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제품 이름
Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli, Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli cleaves asparagine-linked or free oligomannose and hybrid. Suitable for deglycosylation of native proteins.
recombinant
expressed in E. coli
Quality Segment
conjugate
(N-linked)
form
liquid
specific activity
≥16 units/mg protein, ≥17 units/mL
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
foreign activity
Proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
General description
Note: 1 mU = 1 milliunit.
Recombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
Physical form
In 20 mM Tris-HCl, pH 7.5.
Other Notes
One unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.
Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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저장 등급
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable