S9430
SYBR® Green I nucleic acid gel stain
10,000 × in DMSO
Synonym(s):
DNA stain, SYBR® green gel dye, safer gel stain
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About This Item
form
solution
Quality Segment
usage
mL sufficient for 100 mini-gels
greener alternative product characteristics
Designing Safer Chemicals
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sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
General description
SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.
Application
SYBR® Green I nucleic acid gel stain has been used for:
- the quantification of dsDNA
- to stain DNA in polymerase chain reaction (PCR)
- for comet assay technique
- to assess spermatozoon membrane integrity
- for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
- as a fluorescent dye in flow cytometry
- real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA
Features and Benefits
- An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
- It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
- It is less mutagenic than ethidium bromide in Ames tests
- It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
- Useful for many applications with a limited amount of DNA
- The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
- Removal of this stain in-gel digestion and ligation techniques is not needed
- SYBR Green I is a greener alternative product to ethidium bromide for staining
Preparation Note
Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.
Legal Information
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
201.2 °F - closed cup
flash_point_c
94 °C - closed cup
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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