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03978

Methylene Blue solution

concentrate according to Ehrlich, concentrated, aqueous solution

Sinónimos:

Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue

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Fórmula empírica (notación de Hill):
C16H18ClN3S
Número CAS:
61-73-4
Peso molecular:
319.85
NACRES:
NA.47
PubChem Substance ID:
329747853
UNSPSC Code:
12171500
MDL number:
MFCD00012111
Beilstein/REAXYS Number:
3641570
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Quality Segment

100

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

density

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Application

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.
Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

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flash_point_c

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ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Clase de almacenamiento

12 - Non Combustible Liquids

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