I9516
2-Propanol
≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent
Synonym(s):
sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
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About This Item
Product Name
2-Propanol, BioReagent, ≥99.5%, Molecular Biology
grade
Molecular Biology
Quality Level
vapor density
2.1 (vs air)
vapor pressure
33 mmHg ( 20 °C), 44 mmHg ( 25 °C)
product line
BioReagent
assay
≥99.5%
form
liquid
autoignition temp.
750 °F
expl. lim.
2.0-12.7 %, 93 °C
technique(s)
DNA extraction: suitable, RNA extraction: suitable
impurities
≤0.2% water
water
color
colorless
refractive index
n20/D 1.377 (lit.)
bp
82 °C (lit.)
mp
−89.5 °C (lit.)
solubility
water: soluble (completely)
density
0.785 g/mL at 25 °C (lit.)
suitability
suitable for precipitation of nucleic acids
storage temp.
room temp
SMILES string
CC(C)O
InChI
1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
InChI key
KFZMGEQAYNKOFK-UHFFFAOYSA-N
General description
Protocols for the use of isopropanol:
- DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
- Protein crystallization.
- Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.
Application
- in DNA and RNA extraction
- for determination of hydroxyproline levels(The Merck Index, 12th ed)
- for checking cell viability by MTT assay
- in microarray hybridization
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 1
flash_point_f
53.6 °F - closed cup
flash_point_c
12.0 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Protocols
Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Articles
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
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