CS0760
Isolated Mitochondria Staining Kit
1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)
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About This Item
UNSPSC Code:
12352207
EC Number:
217-896-6
NACRES:
NA.32
Technique(s):
flow cytometry: suitable, protein staining: suitable
Usage:
kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)
Storage temp.:
−20°C
Detection method:
fluorometric
Shipped in:
dry ice
Quality Segment
usage
kit sufficient for 1000 reactions (using 96 multiwell plates), kit sufficient for 50 reactions (in a 2 mL cuvette)
packaging
pkg of 1 kit
storage condition
dry at room temperature
technique(s)
flow cytometry: suitable, protein staining: suitable
fluorescence
λex 490 nm; λem 590 nm (JC-1 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
dry ice
storage temp.
−20°C
Application
Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.
Biochem/physiol Actions
The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.
Features and Benefits
- Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
- Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation
Analysis Note
The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.
Kit Components Only
Product No.
Description
- DMSO 1 mL
- JC-1 Assay Buffer 5X 25 mL
- JC-1 Stain 25 μg
- Valinomycin Ready Made 100 μL
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 1 Dermal - Acute Tox. 1 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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