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MABS1259 Anti-CYP51A1 Antibody, clone N6-P2H5*G8

Anti-CYP51A1 Antibody, clone N6-P2H5*G8, Cat. No. MABS1259, is a highly specific mouse monoclonal antibody that targets CYP51A1, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CYP51A1 Antibody, clone N6-P2H5*G8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CYP51A1, CYP51 UniProt Number: Q16850
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MABS1259
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1259
DescriptionAnti-CYP51A1 Antibody, clone N6-P2H5*G8
Alternate Names
  • Lanosterol 14-alpha demethylase
  • CYP51A1
  • CYPL1
  • CYPLI
  • Cytochrome P450 51A1
  • Cytochrome P450, family 51, subfamily A, polypeptide 1
  • Cytochrome P450-14DM
  • Cytochrome P45014DM
  • Cytochrome P450L1
  • Cytochrome P450LI
  • LDM
  • Sterol 14-alpha demethylase
Background InformationLanosterol 14-alpha demethylase (EC 1.14.13.70; UniProt Q16850; also known as CYP51A1, CYPL1, CYPLI, Cytochrome P450 51A1, Cytochrome P450, family 51, subfamily A, polypeptide 1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450L1, Cytochrome P450LI, LDM, Sterol 14-alpha demethylase) is encoded by the CYP51A1 (also known as CYP51) gene (Gene ID 1595) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum where they function as the terminal enzymes in electron transfer chains. In addition to processing endogenous substrates, CYPs also function to metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consisits of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. CYP51A1 represents the only member of the CYP51 subfamily and is involved in the conversion of lanosterol to 4,4-dimethylcholesta-8(9),14,24-trien-3 -ol. Products generated by the CYP51 reaction are vital intermediates in pathways leading to the formation of cholesterol in humans, ergosterol in fungi, and other types of sterols in plants. Sterols play an important structural role in the regulation of membrane fluidity and permeability, which influence the functions of enzymes, ion channels, and other cell components embedded within.
References
Product Information
FormatPurified
PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
Quality SegmentMQ100
Applications
ApplicationAnti-CYP51A1 Antibody, clone N6-P2H5*G8, Cat. No. MABS1259, is a highly specific mouse monoclonal antibody that targets CYP51A1, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: Clone N6-P2H5*G8 hybridoma culture supernatant detected an upregulated CYP51A1 immunoreactivity in human colon carcinoma tissue relative to that in normal liver tissue.

Immunohistochemistry Analysis: A representative lot detected increased CYP51A1 expression in formalin-fixed, paraffin-embedded primary colorectal cancer tissue than in normal colonic mucosa. Significantly reduced CYP51A1 expression was found in lymph node metastasis compared to Dukes C (stage 3) colorectal cancer (Swan, R., et al. (2016). Oncotarget. In press).

Western Blotting Analysis: A representative lot detected a target band in lysate from CYP51A1-transfected, but not from untransfected, cells (Swan, R., et al. (2016). Oncotarget. In press).
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponging to a sequence near the C-terminus of human/mouse/rat CYP51A1.
CloneN6-P2H5*G8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone N6-P2H5*G8 was raised against a CYP51A1 C-terminal sequence 100% conserved among human, mouse, and rat species. Target band was detected only in lysate from CYP51A1-transfected, but not from untransfected, cells (Swan, R., et al. (2016). Oncotarget. In press).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CYP51A1
  • CYP51
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~50 kDa observed. 56.81/46.31 kDa (human isoform 1/2), 56.78 kDa (mouse), and 56.71 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human liver microsome lysate.

Western Blotting Analysis: A 1:125 dilution of this antibody detected CYP51A1 in 50 µg of human liver microsome lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
MABS1259 04054839034992