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AB5150-50UL Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT

Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, C-terminus detects level of Calcium Channel & has been published & validated for use in IC, IP & WB.

Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Signaling Neuroscience Gene Symbol: CACNA1C, TS, MGC120730, CACH2, Cav1.2, CaV1.2, CACNL1A1, CACN2, CCHL1A1 UniProt Number: Q13936
AB5150-50UL
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RICC, IP, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5150-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT
References
Product Information
FormatAffinity Purified
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality SegmentMQ100
Applications
ApplicationAnti-Calcium Channel Antibody, Voltage Gated Pan α 1, C-terminus detects level of Calcium Channel & has been published & validated for use in IC, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesIn preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each) (Westenbroek et al. 1992).

Western blot: 1:200 using ECL on rat brain membranes.

Immunohistochemistry using rat heart sections. Note: the epitope can be masked by other subunits in some Ca2+ channels.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPurified peptide from {C-DNFDYLTRDWSILGPHHLD [CP15], cooresponding to residues 1506-1524 of rat alpha-1C {Accession P2202} subunit of skeletal muscle voltage-gated calcium channel (Striessnig et al. 1990), conjugated to KLH (Harlow & Lane 1988).
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes all types of alpha1 subunits of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. The epitope recongnized by the AB5150 is masked by alpha2 and delta chains, so this antibody can bind only after using Triton X 100 or other detergents that cause subunit dissociation (Ahlijanian et al. 1991).
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Gene Symbol
  • CACNA1C
  • TS
  • MGC120730
  • CACH2
  • Cav1.2
  • CaV1.2
  • CACNL1A1
  • CACN2
  • CCHL1A1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13936 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the high-voltage activated (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin- GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds.
SIZE: 2221 amino acids; 248887 Da
SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with CACNA2D4.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
DOMAIN: SwissProt: Q13936 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. & Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel (By similarity).
PTM: Phosphorylation by PKA activates the channel (By similarity).
DISEASE: SwissProt: Q13936 # Defects in CACNA1C are the cause of Timothy syndrome (TS) [MIM:601005]. TS is a disorder characterized by multiorgan dysfunction including lethal arrhythmias, webbing of fingers and toes, congenital heart disease, immune deficiency, intermittent hypoglycemia, cognitive abnormalities and autism.
SIMILARITY: Belongs to the calcium channel alpha-1 subunit family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB5150-50UL 04053252740039

Documentation

Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT SDS

Title

Safety Data Sheet (SDS) 

Anti-Calcium Channel Antibody, Voltage Gated Pan α 1, CT Certificates of Analysis

TitleLot Number
RABBIT ANTI-PAN #945;1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2144934 2144934
RABBIT ANTI-PAN a1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2048751 2048751
RABBIT ANTI-PAN a1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2052340 2052340
RABBIT ANTI-PAN a1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2228072 2228072
RABBIT ANTI-PAN a1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2299234 2299234
RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL 2483198
RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2421544 2421544
RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL - 3231978 3231978
RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY 3052517
RABBIT ANTI-PAN α1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY 2995964
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References

Reference overviewPub Med ID
Immunochemical indentification and subcellular distribution of the alpha 1A subunits of brain calcium channels.
Westenbroek, R.E. et al.
J. Neurosci., 15:6403-6418 (1995)  1995

7472404 7472404

Data Sheet

Title
RABBIT ANTI-PAN 1 VOLTAGE GATED CALCIUM CHANNEL AFFINITY PURIFIED POLYCLONAL ANTIBODY