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MAC133 Anti-Cas9 Antibody, clone 7A9

Anti-Cas9 Antibody, clone 7A9 | MAC133 is an antibody against Cas9 Antibody for use in Western Blotting, Immunoprecipitation and Immunocytochemistry.

Anti-Cas9 Antibody, clone 7A9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies Research Sub-Category: Secondary Antibodies Adsorbed for Dual Labeling
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MAC133
100 µg  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAC133
      DescriptionAnti-Cas9 Antibody, clone 7A9
      Alternate Names
      • dCas9
      Background InformationCas9 is an essential endonuclease in Streptococcus pyogenes serotype M1 that is part of that organism’s innate immunity system, or CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or to help distinguish self versus nonself. Researchers hope to exploit the programmability and precise nature of Cas9 cleavage for more effective recombinant DNA technologies.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationAnti-Cas9 Antibody, clone 7A9 | MAC133 is an antibody against Cas9 Antibody for use in Western Blotting, Immunoprecipitation and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Cas9 in 1 µg of Myc-Cas9 expressing HEK293 cell lysate.
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Cas9 in Flag-Cas9 and Myc-Cas9 expressing cell lysate (performed by an independent laboratory).
      Biological Information
      ImmunogenN-Terminal domain of Cas9.
      Clone7A9
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityMAC133 recognises both Cas9 and dCas9 (nuclease deficient Cas9)
      IsotypeIgG1κ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Molecular Weight~160 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 expressing Flag-Cas9 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Cas9 in 1 µg of HEK293 cell lysate expressing Flag-Cas9.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MAC133 04055977127034

      Documentation

      Anti-Cas9 Antibody, clone 7A9 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cas9 Antibody, clone 7A9 Certificates of Analysis

      TitleLot Number
      Anti-Cas9, clone 7A9 3167596
      Anti-Cas9, clone 7A9 - 2532575 2532575
      Anti-Cas9, clone 7A9 - 3205439 3205439
      Anti-Cas9, clone 7A9 - 3256668 3256668
      Anti-Cas9, clone 7A9 - 3313137 3313137
      Anti-Cas9, clone 7A9 - 3482825 3482825
      Anti-Cas9, clone 7A9 - 3681278 3681278
      Anti-Cas9, clone 7A9 - 3835371 3835371
      Anti-Cas9, clone 7A9 - 3874277 3874277
      Anti-Cas9, clone 7A9 - 3915598 3915598
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