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IF14 Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10)

Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~45 kDa cytokeratin 18 protein in a majority of adenocarcinoma and aductal carcinomas, but not squamos cell carcinomas.
      Catalogue NumberIF14
      Brand Family Calbiochem®
      Application Data
      Detection of human cytokeratin 18 by immunohistochemistry. Sample: Human breast cancer tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: fluorescence.

      Detection of human Cytokeratin 18 by immunoblotting. Sample: Human cytokeratin 18. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesCarley, W.W., et al. 1996. Microcirculation 3, 359.
      Herzig, K.H., et al. 1994. Gastroenterology 106 1326.
      Rafiee, P., et al. 1992. 7, 123.
      Dodge, A.B., et al. 1991. Comp. Biochem. Physiol. 98, 461.
      Patton, W.F., et al. 1990. J. Cell. Physiol. 143, 140.
      Stosiek, P., et al. 1990. Acta Histochem. 89, 61.
      Chung-Welch, N., et al. 1989. Differentiation 42, 44.
      Jahn, L., et al. 1987. Differentiation 36, 234.
      Moll, R., et al. 1983. Differentiation 23, 256.
      Geisler, N., et al. 1982. Cell 30, 277.
      Lazarides, E., 1982. Ann. Rev. Biochem. 51, 219.
      Moll, R., et al. 1982. Cell 31, 11.
      Osborn, M. and Weber, K., 1982. Cell 31, 303.
      Fuchs, E.V. and Coppock, S.M. 1981. Cell 27, 75.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Negative controlArterial blood vessels or HUVECs
      Positive controlHeLa cells, mesothelial cells, or skin or hepatocellular carcinoma tissue
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (1-2 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Immunofluorescence (see comments)
      Paraffin Sections (1-2 µg/ml, pressure cooker pre-treatment required)
      Application CommentsThis antibody reacts with a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia. It has been reported that tissues from the gastrointestinal tract are positive for both CK8 and CK18 but do not contain CK14. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenhuman PMC42 breast cancer cells
      ImmunogenHuman
      CloneDC10
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      IF14 0

      Documentation

      Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) Certificates of Analysis

      TitleLot Number
      IF14

      References

      Reference overview
      Carley, W.W., et al. 1996. Microcirculation 3, 359.
      Herzig, K.H., et al. 1994. Gastroenterology 106 1326.
      Rafiee, P., et al. 1992. 7, 123.
      Dodge, A.B., et al. 1991. Comp. Biochem. Physiol. 98, 461.
      Patton, W.F., et al. 1990. J. Cell. Physiol. 143, 140.
      Stosiek, P., et al. 1990. Acta Histochem. 89, 61.
      Chung-Welch, N., et al. 1989. Differentiation 42, 44.
      Jahn, L., et al. 1987. Differentiation 36, 234.
      Moll, R., et al. 1983. Differentiation 23, 256.
      Geisler, N., et al. 1982. Cell 30, 277.
      Lazarides, E., 1982. Ann. Rev. Biochem. 51, 219.
      Moll, R., et al. 1982. Cell 31, 11.
      Osborn, M. and Weber, K., 1982. Cell 31, 303.
      Fuchs, E.V. and Coppock, S.M. 1981. Cell 27, 75.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      ApplicationFrozen Sections (1-2 µg/ml)
      Immunoblotting (1-2 µg/ml)
      Immunofluorescence (see comments)
      Paraffin Sections (1-2 µg/ml, pressure cooker pre-treatment required)
      Application Data
      Detection of human cytokeratin 18 by immunohistochemistry. Sample: Human breast cancer tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: fluorescence.

      Detection of human Cytokeratin 18 by immunoblotting. Sample: Human cytokeratin 18. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3 X63.Ag8.653 mouse myeloma cells. Recognizes the ~45 kDa cytokeratin 18 protein.
      BackgroundIntermediate (10 nm) filaments (IFs) are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (GFA), skeletal, visceral and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of ~40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 18 (CK18) is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 exists in combination with CK8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only cytokeratins 8 and 18.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman PMC42 breast cancer cells
      CloneDC10
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa cells, mesothelial cells, or skin or hepatocellular carcinoma tissue
      Negative controlArterial blood vessels or HUVECs
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody reacts with a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia. It has been reported that tissues from the gastrointestinal tract are positive for both CK8 and CK18 but do not contain CK14. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesCarley, W.W., et al. 1996. Microcirculation 3, 359.
      Herzig, K.H., et al. 1994. Gastroenterology 106 1326.
      Rafiee, P., et al. 1992. 7, 123.
      Dodge, A.B., et al. 1991. Comp. Biochem. Physiol. 98, 461.
      Patton, W.F., et al. 1990. J. Cell. Physiol. 143, 140.
      Stosiek, P., et al. 1990. Acta Histochem. 89, 61.
      Chung-Welch, N., et al. 1989. Differentiation 42, 44.
      Jahn, L., et al. 1987. Differentiation 36, 234.
      Moll, R., et al. 1983. Differentiation 23, 256.
      Geisler, N., et al. 1982. Cell 30, 277.
      Lazarides, E., 1982. Ann. Rev. Biochem. 51, 219.
      Moll, R., et al. 1982. Cell 31, 11.
      Osborn, M. and Weber, K., 1982. Cell 31, 303.
      Fuchs, E.V. and Coppock, S.M. 1981. Cell 27, 75.