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MAB2623 Anti-EMMPRIN [CD147] Antibody, clone 1S9-2A

This Anti-EMMPRIN [CD147] Antibody, clone 1S9-2A is validated for use in WB, IH for the detection of EMMPRIN [CD147].

Anti-EMMPRIN [CD147] Antibody, clone 1S9-2A MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: MMPs & TIMPs Gene Symbol: BSG UniProt Number: P35613
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MAB2623
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2623
      DescriptionAnti-EMMPRIN [CD147] Antibody, clone 1S9-2A
      Alternate Names
      • Basigin
      • CD147 antigen
      • Collagenase stimulatory factor
      • Extracellular matrix metalloproteinase inducer
      • Leukocyte activation antigen M6
      • OK blood group antigen
      • Tumor cell-derived collagenase stimulatory factor
      • TCSF
      Background InformationMatrix metalloproteinases (MMPs) or gelatinases play an important role in the early invasion of malignant tumors. These enzymes may be released directly from tumor cells, or may be derived from fibroblasts that have been stimulated with the extracellular MMP-inducer EMMPRIN. Studies have demonstrated that EMMPRIN stimulates human fibroblast production of interstitial collagenase, stromelysin-1, and gelatinase A (MMP-2), which is essential for tissue degradation and remodeling during tumor invasion and wound healing.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationThis Anti-EMMPRIN [CD147] Antibody, clone 1S9-2A is validated for use in WB, IH for the detection of EMMPRIN [CD147].
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImunohistochemistry Analysis: A 1:100 dilution from a previous lot detected EMMPRIN in human adenocarcinoma tissue.
      Biological Information
      ImmunogenHuman synovial fibroblasts.
      EpitopeUnknown
      Clone1S9-2A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody recognizes EMMPRIN.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a plasma membrane protein that is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. The encoded protein is also a member of the immunoglobulin superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • BSG
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes (By similarity).

      SIZE: 385 amino acids; 42200 Da

      SUBUNIT: Forms homo-oligomers in a cis-dependent manner on the plasma membrane. Forms a complex with MMP1 at the tumor cell surface. Interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with ATP1B2, MAG and L1CAM (By similarity). Interacts with AJAP1.

      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). Melanosome. Note=Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

      TISSUE SPECIFICITY: Present only in vascular endothelium in non- neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

      PTM: N-glycosylated.

      SIMILARITY: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Molecular Weight~ 35-58 kDa. The extent of CD147 glycosylation is tissue-specific and is responsible for a variation in the apparent molecular mass from 35 kDa to 58 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.1 µg/ml of this antibody detected EMMPRIN on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MAB2623 04053252576829

      Documentation

      Anti-EMMPRIN [CD147] Antibody, clone 1S9-2A SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-EMMPRIN [CD147] Antibody, clone 1S9-2A Certificates of Analysis

      TitleLot Number
      Anti-EMMPRIN [CD147], clone 1S9-2A - 2370705 2370705
      Anti-EMMPRIN [CD147], clone 1S9-2A - 2455736 2455736
      Anti-EMMPRIN [CD147], clone 1S9-2A - 2262741 2262741
      Anti-EMMPRIN [CD147], clone 1S9-2A - 3071656 3071656
      Anti-EMMPRIN [CD147], clone 1S9-2A - 3954466 3954466
      Anti-EMMPRIN [CD147], clone 1S9-2A - NG1750559 NG1750559
      Anti-EMMPRIN [CD147], clone 1S9-2A - NG1872066 NG1872066
      Anti-EMMPRIN [CD147], clone 1S9-2A - NRG1675585 NRG1675585
      Anti-EMMPRIN [CD147], clone 1S9-2A -2506452 2506452
      Anti-EMMPRIN [CD147], clone 1S9-2A -2701000 2701000