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CB1007 Anti-GroEL Mouse mAb (9A1/2)

Anti-GroEL Mouse mAb (9A1/2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CB1007
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~60 kDa GroEL protein from E. coli and Pseudomonas.
Catalogue NumberCB1007
Brand Family Calbiochem®
Application Data
Detection of E. coli GroEL by immunoblotting. Samples: Recombinant GroEL bacterial lysate from protein (lane 1), recombinant Hsp60 protein (negative control) (lane 2), and E. coli (lane 3). Primary antibody: Anti-GroEL Mouse mAb (9A1/2) (Cat. No. CB1007) (1:1000). Detection: chemiluminescence.
References
ReferencesDukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746.
Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol.
Positive controlE. coli lysate
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunoblotting Dukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746. Immunoprecipitation Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:5000, colorimetric; see application references)
Immunoprecipitation (1:250; see application references)
Application CommentsDoes not cross-react with eukaryotic Hsp60, a GroEL homolog. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenGroEL purified from an overproducing strain of E. coli
ImmunogenE. coli
Clone9A1/2
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36-A09

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
CB1007 0

Documentation

Anti-GroEL Mouse mAb (9A1/2) Certificates of Analysis

TitleLot Number
CB1007

References

Reference overview
Dukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746.
Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-June-2008 JSW
ApplicationImmunoblotting (1:5000, colorimetric; see application references)
Immunoprecipitation (1:250; see application references)
Application Data
Detection of E. coli GroEL by immunoblotting. Samples: Recombinant GroEL bacterial lysate from protein (lane 1), recombinant Hsp60 protein (negative control) (lane 2), and E. coli (lane 3). Primary antibody: Anti-GroEL Mouse mAb (9A1/2) (Cat. No. CB1007) (1:1000). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~60 kDa bacterial chaperonin GroEL protein.
BackgroundGroEL functions in prokaryotic protein folding and assembly in an ATP-dependent manner with another protein of the groE operon, GroES. In vitro, groE gene products have been demonstrated to modulate folding of a number of eukaryotic proteins. GroEL is essential for morphogenesis of bacteriophage lambda, where it mediates assembly of phage head and tail protein complexes. GroEL homologs have been identified in plant chloroplasts and in the mitochondria of a variety of eukaryotes (Hsp60), where in conjunction with Hsp70, they participate in the folding and assembly of proteins translocated into the matrix. Recently a chaperone protein analogous to GroEL was identified in the eukaryotic cytosol, TCP-1, raising the possibility of Hsp60 and Hsp70 cooperation in the maturation of cytosolic proteins.
HostMouse
Immunogen speciesE. coli
ImmunogenGroEL purified from an overproducing strain of E. coli
Clone9A1/2
IsotypeIgG₁
SpeciesE. coli
Positive controlE. coli lysate
FormLiquid
FormulationIn PBS, 50% glycerol.
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with eukaryotic Hsp60, a GroEL homolog. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesDukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746.
Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
Application referencesImmunoblotting Dukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746. Immunoprecipitation Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.