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DR1016 Anti-H2AX Rabbit pAb

Anti-H2AX Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Histone H2AX

DR1016
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form in 293T and A431 cell nuclear extracts.
Catalogue NumberDR1016
Brand Family Calbiochem®
SynonymsAnti-Histone H2AX
Application Data
Detection of human H2AX by immunoblotting. Sample: Nuclear extract from HEK293T cells, H2AX knockout (-/-), H2AX wild-type (+/+), and G-361 cells. Primary antibody: Anti-H2AX Rabbit pAb (Cat. No. DR1016) (1:1000). Detection: chemiluminescence.
References
ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
Product Information
FormLiquid
FormulationIn Tris/citrate-phosphate buffer, pH 7-8.
Positive controlNuclear extracts from human 293T or G-361 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:5000-1:15,000)
Immunoprecipitation (5-20 µg/mg protein from lysate)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human H2AX
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Highly Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
DR1016 0

Documentation

Anti-H2AX Rabbit pAb Certificates of Analysis

TitleLot Number
DR1016

References

Reference overview
Stiff. T., et al. 2004. Cancer Res. 64, 2390.
Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
SynonymsAnti-Histone H2AX
ApplicationImmunoblotting (1:5000-1:15,000)
Immunoprecipitation (5-20 µg/mg protein from lysate)
Application Data
Detection of human H2AX by immunoblotting. Sample: Nuclear extract from HEK293T cells, H2AX knockout (-/-), H2AX wild-type (+/+), and G-361 cells. Primary antibody: Anti-H2AX Rabbit pAb (Cat. No. DR1016) (1:1000). Detection: chemiluminescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquitinated form.
BackgroundThe nucleosome is composed of ~200 bp of DNA and eight histone proteins, two from each of the four histone protein families, H4, H3, H2B, and H2A. H2A includes three subfamilies: H2A1-H2A2, H2AZ, and H2AX (LocusLink ID 3014), which have been conserved throughout eukaryotic evolution. Phosphorylation of H2AX on Ser139 is an early step in the response to DNA damage induced by ionizing radiation. Phosphorylation of H2AX is required for the accumulation of 53BP1 at DNA break sites.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids at the C-terminus of human H2AX
IsotypeIgG
Specieshuman, mouse
Positive controlNuclear extracts from human 293T or G-361 cells
FormLiquid
FormulationIn Tris/citrate-phosphate buffer, pH 7-8.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
Toxicity Highly Toxic
ReferencesStiff. T., et al. 2004. Cancer Res. 64, 2390.
Taneja, N., et al. 2004. J. Biol. Chem. 279, 2273.
Ward, I.M., et al. 2003. J. Biol. Chem. 278, 19579.
Rogakou, E.P., et al. 1998. J. Biol. Chem. 273, 5858.