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ABD113-25UG Anti-HLA-DRB3

Detect HLA-DRB3 using this rabbit polyclonal Anti-HLA-DRB3 Antibody, Cat. No. ABD113-25UG, validated for use in Western Blotting and Flow Cytometry.

Anti-HLA-DRB3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: HLA-DRB3 UniProt Number: P79483
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ABD113-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABD113-25UG
      DescriptionAnti-HLA-DRB3
      Alternate Names
      • HLA class II histocompatibility antigen
      • DR beta 3 chain
      • MHC class II antigen DRB3
      Background InformationHLA class II histocompatibility antigen, DR beta 3 chain (UniProt: P79483; also known as MHC class II antigen DRB3) is encoded by the HLA-DRB3 gene (Gene ID: 3125) in human. DRB3 is a single-pass type I membrane protein; that is synthesized with a signal peptide of 29 amino acids that is subsequently cleaved to generate a mature form. It also contains an Ig0like C1 type domain (aa 126-214). It is a heterodimer of an alpha and a beta subunit; also referred as MHC class II molecule. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the endoplasmic reticulum (ER) to form a heterononamer. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. DRB3 binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationDetect HLA-DRB3 using this rabbit polyclonal Anti-HLA-DRB3 Antibody, Cat. No. ABD113-25UG, validated for use in Western Blotting and Flow Cytometry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 1 µg from a representative lot detected HLA-DRB3 in one million Raji cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human HLA class II histocompatibility antigen, DR beta 3 chain (HLA-DRB3).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects HLA class II histocompatibility antigen, DR beta 3 chain in human cells. It targets an epitope within 15 amino acids from the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • HLA-DRB3
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight32 kDa kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.
      Western Blotting Analysis: 2 µg/mL of this antibody detected HLA-DRB3 in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      ABD113-25UG 04054839453762