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ABS55 Anti-InsP3R Antibody, Type 1

This Anti-InsP3R Antibody, Type 1 is validated for use in WB, IP, IH(P) for the detection of InsP3R.

Anti-InsP3R Antibody, Type 1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: ITPR1, IP3R1, Insp3r1, InsP3R1, IP3R, INSP3R1, SCA15 UniProt Number: P29994
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ABS55
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M, Po, Pm, Rhesus MacaqueWB, IP, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS55
      DescriptionAnti-InsP3R Antibody, Type 1
      Alternate Names
      • inositol 1,4,5-triphosphate receptor, type 1
      • spinocerebellar ataxia 16
      • inositol 1,4,5-trisphosphate receptor type 1
      • Type 1 inositol 1,4,5-trisphosphate receptor
      • Type 1 InsP3 receptor
      • IP3 receptor
      • spinocerebellar ataxia 15
      • IP3 receptor isoform 1
      Background InformationThe inositol (1,4,5)-trisphosphate receptor (InsP3R) regulates calcium release from storage within cells and plays an important role in neuronal function. InsP3R has been linked to Huntington’s disease. InsP3R has also been linked to the association between cAMP and Ca2+ signaling in regions of the brain, including the neostriatum.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat brain microsomal tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationThis Anti-InsP3R Antibody, Type 1 is validated for use in WB, IP, IH(P) for the detection of InsP3R.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: 1:1500 dilution from a previous lot detected InsP3R in purkinje cells at the junction of the granular and molecular layers of normal rat cerebellum tissue.

      Immunoprecipitation Analysis: 10 µg of Anti-InsP3R, Type 1 immunoprecipitated InsP3R, Type 1 from rat brain microsomal prep RIPA lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain of rat InsP3R.
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes InsP3R at the cytoplasmic domain.
      Species Reactivity
      • Rat
      • Human
      • Mouse
      • Pig
      • Primate
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Human and Rat. Predicted to react with Mouse, Porcine, Primate, and Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene Summaryreceptor for inositol 1,4,5-triphosphate; mediates a IP3-dependent calcium influx [RGD].
      Gene Symbol
      • ITPR1
      • IP3R1
      • Insp3r1
      • InsP3R1
      • IP3R
      • INSP3R1
      • SCA15
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate.

      SUBUNIT STRUCTURE: Homotetramer. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Interacts with CABP1. Interacts with AHCYL1 and MRVI1 (By similarity).

      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.

      TISSUE SPECIFICTY: Isoform 3 is found in the brain while isoform 7 is found in the fetal brain and peripheral tissues.

      DOMAIN: The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.

      PTM: Phosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.

      Phosphorylated on tyrosine residues (By similarity).

      MISCELLANEOUS: Calcium appears to inhibit ligand binding to the receptor, most probably by interacting with a distinct calcium-binding protein which then inhibits the receptor.

      SEQUENCE SIMILARITIES: Belongs to the InsP3 receptor family.

      Contains 5 MIR domains.
      Molecular Weight~300 kDa observed. UniProtKB/Swiss-Prot P29994 (ITPR1_RAT) entry describes 8 isoforms produced by alternative splicing: Isoform 1 at 313.264 kDa, Isoform 2 at 311.498 kDa, Isoform 3 at 313.136 kDa, Isoform 4 at 311.370 kDa, Isoform 5 at 311.210 kDa, Isoform 6 at 309.444 kDa, Isoform 7 at 308.674 kDa, and Isoform 8 at 306.909 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain microsomal tissue lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected InsP3R on 10 µg of rat brain microsomal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      ABS55 04053252520501

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-InsP3R Antibody, Type 1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-InsP3R Antibody, Type 1 Certificates of Analysis

      TitleLot Number
      Anti-InsP3R, Type 1 2478034
      Anti-InsP3R, Type 1 - 2426541 2426541
      Anti-InsP3R, Type 1 - 2279576 2279576
      Anti-InsP3R, Type 1 - 3189226 3189226
      Anti-InsP3R, Type 1 - 3892220 3892220
      Anti-InsP3R, Type 1 - 4159509 4159509
      Anti-InsP3R, Type 1 - NG1949598 NG1949598
      Anti-InsP3R, Type 1 - NRG1855198 NRG1855198
      Anti-InsP3R, Type 1 -2596520 2596520
      Anti-InsP3R, Type 1 -2604240 2604240
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