Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS2287-100UG Anti-Jagged2 Antibody, clone HMJ2-1

Anti-Jagged2, clone HMJ2-1, Cat. No. MABS2287-100UG is an Armenian hamster monoclonal antibody that detects Jagged2 and is tested for use in Flow Cytometry, Agonist, and ELISA.

NewMABS2287-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2287-100UG
DescriptionAnti-Jagged2 Antibody, clone HMJ2-1
Alternate Names
  • D12Ggc2e
  • JAG2
  • mJagged2-1
  • Protein jagged-2
  • syndactylism
Background InformationProtein jagged-2 (UniProt: Q9QYE5; also known as Jagged2) is encoded by the Jag2 gene (Gene ID:16450) in mouse. The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch protein family are transmembrane receptors that are critical for various cell fate decisions. Jagged2 is one of several ligands that activate Notch and related receptors. It is a single-pass type I membrane protein and is synthesized with a signal peptide sequence (aa 1-23), the removal of which yields the mature Jagged2 with a large extracellular domain (aa 24-1082), a transmembrane domain (aa 1083-1103) and, a cytoplasmic domain (aa 1104-1247). Jagged2 expression varies across different tissues and developmental stages and is found to be highest in fetal thymus, epidermis, foregut dorsal root ganglia and inner ear. In 2-weeK-old mice its abundantly found in heart, lung, thymus, skeletal muscle, brain and testis. Its expression overlaps partially with Notch1 expression. Jagged2 has been implicated in various diseases, mainly due to its role in the Notch signaling pathway. The interaction of Natural Killer (NK) cells with dendritic cells (DC) contributes to NK cell-mediated antitumor or antimicrobial responses. Studies show an involvement of Jagged2 Notch interaction in augmenting NK cell cytotoxicity mediated by dendritic cells and indicated that, the Jagged2 Notch axis plays a crucial role in DC-mediated NK cell cytotoxicity. It is also suggested that manipulation of this interaction may provide an approach to induce potent tumor immunity or to inhibit certain autoimmune diseases caused by NK cell activation. This clone functions as an agonist by promoting Treg cell differentiation. Aberrant Jagged2 expression can lead to uncontrolled cell proliferation and tumorigenesis in cancers such as breast cancer, lung cancer, and glioblastoma. (Ref.: Kijima, M., et al. (2008). Proc Natl Acad Sci USA. 105(19):7010-5; Elyaman, W., et al. (2012). Immunity. 36(4):623-34; Riella, L.V., et al. (2013). Eur J Immunol. 43(6):1449-58; Sekine, C., et al. (2014). Arthritis Rheumatol. 66(10):2751-61).
References
Product Information
FormatPurified
PresentationPurified Armenian hamster monoclonal antibody in PBS without azide.
Quality SegmentMQ200
Applications
ApplicationAnti-Jagged2, clone HMJ2-1, Cat. No. MABS2287-100UG is an Armenian hamster monoclonal antibody that detects Jagged2 and is tested for use in Flow Cytometry, Agonist, and ELISA.
Key Applications
  • Flow Cytometry
  • ELISA
  • Agonist or Inhibitor
Application NotesTested Applications

Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected Jagged2 in CHO cells transfected with mouse Jagged2 (Courtesy of Dr. Hideo Yagita, Ph.D., Juntendo University, Japan).

ELISA: A representative lot detected Jagged2 in ELISA application (Sekine, C., et al. (2014). Arthritis Rheumatol. 66(10):2751-61).

Flow Cytometry Analysis: A representative lot detected Jagged2 in Flow Cytometry application (Kijima, M., et al. (2008). Proc Natl Acad Sci USA. 105(19):7010-5).

Agonist: A representative lot promoted Treg cell differentiation and enhanced Jagged-2 Notch signaling via activation of RBP-Jk (canonical Notch pathway). (Elyaman, W., et al. (2012). Immunity. 36(4):623-34; Riella, L.V., et al. (2013). Eur J Immunol. 43(6):1449-58).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenCHO cells overexpressing mouse Jagged2
EpitopeExtracellular domain
CloneHMJ2-1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostArmenian Hamster
SpecificityClone HMJ2-1 is an Armenian Hamster monoclonal antibody that specifically detects Jagged2.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Jag2
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight; 134.75 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in CHO cells transfected with mouse Jagged2.

Flow Cytometry Analysis: 1.0 g of this antibody detected Jagged2 in one million CHO cells transfected with mouse Jagged2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
MABS2287-100UG 04065272290371

Related Products & Applications