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MAB88918 Anti-Laminin Antibody, clone 2G6/A2

This Anti-Laminin Antibody, clone 2G6/A2 is validated for use in ELISA, IP, IC, IH for the detection of Laminin.

Anti-Laminin Antibody, clone 2G6/A2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: LAMB1, MGC142015, CLM UniProt Number: P07942
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MAB88918
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, IP, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB88918
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Laminin Antibody, clone 2G6/A2
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 0.01 M Phosphate buffer, 0.15 M NaCl, pH 7.1, sterile filtered.
      Quality SegmentMQ100
      Applications
      ApplicationThis Anti-Laminin Antibody, clone 2G6/A2 is validated for use in ELISA, IP, IC, IH for the detection of Laminin.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry: (5-20 μg/mL)

      ELISA: (5-20 μg/mL)

      Immunohistochemistry: (5-20 μg/mL) Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPepsin fragments of human laminin from placenta.
      Clone2G6/A2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with human laminin both in ELISA and on cells and frozen sections. It is able to precipitate laminin from the culture medium. The antibody does not inhibit neurite outgrowth from neurons induced by laminin as well as the binding of heparin to laminin. It does not react with laminin from rat and mouse.The binding of the antibody to human laminin is not inhibited by the laminin-pentapeptide Tyr-Ile-Gly-Ser-Arg (Iwanmoto, 1987).The staining of laminin was tested on a number of materials including human lung fibroblasts (IMR 90) and human skeletal muscle tissue.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLaminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
      Gene Symbol
      • LAMB1
      • MGC142015
      • CLM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07942 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
      SIZE: 1786 amino acids; 198066 Da
      SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Beta-1 is a subunit of laminin-1 (EHS laminin), laminin-2 (merosin), and laminin-6 (K-laminin).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
      DOMAIN: SwissProt: P07942 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI and IV are globular.
      SIMILARITY: Contains 13 laminin EGF-like domains. & Contains 1 laminin IV type B domain. & Contains 1 laminin N-terminal domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MAB88918 04053252505577

      Documentation

      Anti-Laminin Antibody, clone 2G6/A2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Laminin Antibody, clone 2G6/A2 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-LAMININ - 3384543 3384543
      MOUSE ANTI-LAMININ - 3873572 3873572
      MOUSE ANTI-LAMININ -2790705 2790705
      MOUSE ANTI-LAMININ MONOCLONAL ANTIBODY 2882690
      MOUSE ANTI-LAMININ MONOCLONAL ANTIBODY 3041045
      MOUSE ANTI-LAMININ MONOCLONAL ANTIBODY - 3172597 3172597
      MOUSE ANTI-LAMININ MONOCLONAL ANTIBODY -2521190 2521190
      MOUSE ANTI-LAMININ MONOCLONAL ANTIBODY -2667335 2667335
      MOUSE ANTI-LAMININ MONOCLONAL ANTIBODY -2721556 2721556

      References

      Reference overviewPub Med ID
      Adhesion of human monocytic THP-1 cells to endothelial cell adhesion molecules or extracellular matrix proteins via beta1 integrins regulates heparin binding epidermal growth factor-like growth factor (HB-EGF) expression
      Mograbi, B, et al
      Eur Cytokine Netw, 10:79-86 (1999)  1999

      		10210777 10210777

      Data Sheet

      Title
      MOUSE ANTI-LAMININ

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      Categories

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