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AB9770-200UL Anti-MaxiK Antibody, aa1184-1200.

Detect MaxiK using this Anti-MaxiK Antibody, aa1184-1200. validated for use in IH, IP & WB.

Anti-MaxiK Antibody, aa1184-1200. MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNMA, KCNMA1, SLO1, KCa1.1, MAXIK UniProt Number: Q08460, Q12791
AB9770-200UL
200 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB9770-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MaxiK Antibody, aa1184-1200.
      Alternate Names
      • KCA1.1
      • BKCA
      • Large conductance Ca2+-activated K+ channel
      • BKCa
      • Maxi K+
      • KCNMA1
      References
      Product Information
      FormatAffinity Purified
      Control
      • Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality SegmentMQ100
      Applications
      ApplicationDetect MaxiK using this Anti-MaxiK Antibody, aa1184-1200. validated for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern blot: 1:500 using ECL on rat brain membranes.

      Immunohistochemistry: On rat brain sections.

      Immunoprecipitation: 4 μg using rat brain lysate.

      Dilutions should be made using a carrier protein such as BSA (1-3%).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide from amino acids 1184-1200 of mouse KCa1.1 .
      EpitopeC-terminal intracellular region, STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes MaxiK, amino acids 1184-1200 (KCA1.1, BKCA, Large conductance Ca2+-activated K+ channel, BKCa, Maxi K+, KCNMA1).
      Species Reactivity
      • Rat
      Species Reactivity NoteThe immunogen sequence is identical in human, bovine, chicken, and 16 of 17 amino acids identical in dog.
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryMaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
      Gene Symbol
      • KCNMA
      • KCNMA1
      • SLO1
      • KCa1.1
      • MAXIK
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q12791 # Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).


      SIZE: 1236 amino acids; 137560 Da

      SUBUNIT: Homotetramer; which constitutes the calcium-activated potassium channel. Interacts with beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. Beta subunits are accessory, and modulate its activity.

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

      TISSUE SPECIFICITY: Widely expressed. Except in myocytes, it is almost ubiquitously expressed.


      DOMAIN: SwissProt: Q12791 The S0 segment is essential for the modulation by the accessory beta subunits KCNMB1, KCNMB2, KCNMB3 and KCNMB4. & The S4 segment, which is characterized by a series of positively charged amino acids at every third position, is part of the voltage-sensor. & The pore-forming domain (also referred as P region) is imbedded into the membrane, and forms the selectivity filter of the pore. It contains the signature sequence of potassium channels that displays selectivity to potassium. & The RCK N-terminal domain mediates the homotetramerization, thereby promoting the assembly of monomers into functional potassium channel. It includes binding sites for Ca(2+) and Mg(2+) (By similarity). & The calcium bowl constitutes one of the Ca(2+) sensors and probably acts as a Ca(2+)-binding site. There are however other Ca(2+) sensors regions required for activation of the channel.

      PTM: Phosphorylated (Probable). Phosphorylation by kinases such as PKA and/or PKG. In smooth muscles, phosphorylation affects its activity.

      DISEASE: SwissProt: Q12791 # Defects in KCNMA1 are the cause of generalized epilepsy and paroxysmal dyskinesia (GEPD) [MIM:609446]. Epilepsy is one of the most common and debilitating neurological disorders, affecting more than 40 million people worldwide. Paroxysmal dyskinesias are another heterogeneous group of neurological disorders characterized by sudden, unpredictable, disabling attacks of involuntary movement often requiring life-long treatment. The coexistence of epilepsy and paroxysmal dyskinesia in the same individual or family is an increasingly recognized phenomenon. The basic pathophysiology underlying the coexistence of these two disorders is caused by mutations in the KCNMA1 gene.

      SIMILARITY: Belongs to the potassium channel family. Calcium- activated subfamily. & Contains 1 RCK N-terminal domain.

      MISCELLANEOUS: The protein was initially thought to contain two functionally distinct parts: The core channel (from the N-terminus to the S9 segment) that mediates the channel activity, and the cytoplasmic tail (from the S9 segment to the C-terminus) that mediates the calcium sensing. The situation is however more complex, since the core channel also contains binding sites for Ca(2+) and Mg(2+).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      AB9770-200UL 04053252733673

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies