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MABF2751-25UL Anti-Murine Coronavirus MHV-JHM nucleocapsid Antibody, clone 1-16-1

Anti-Murine Coronavirus MHV-JHM nucleocapsid, clone 1-16-1, Cat. No. MABF2751, mouse monoclonal antibody, detects Murine Coronavirus MHV-JHM nucleocapsid and is tested in Immunofluorescence, Immunohistochemistry, Immunoprecipitation & Western Blotting.

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MABF2751-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2751-25UL
      DescriptionAnti-Murine Coronavirus MHV-JHM nucleocapsid Antibody, clone 1-16-1
      Alternate Names
      • Nucleoprotein
      • Nucleocapsid protein
      • NC
      • Protein N
      Background InformationNucleoprotein (UniProt: C0KYZ4; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene(Gene ID: 1489757) in Murine coronavirus (MHV-JHM.IA). The murine coronavirus mouse hepatitis virus (MHV), a member of the Coronaviridae family, is an enveloped virus with a single-stranded, positive-sense RNA genome. The MHV-JHM strain is shown to be highly neurovirulent and can spread in the brain in mCEACAM1a independent manner. MHV-JHM infection in susceptible weanling mice is reported to result in an encephalomyelitis when neurons, astrocytes, and oligodendrocytes are infected. MHV infection of the murine central nervous system has also been used as a model for the study of chronic demyelinating diseases. The nucleocapsid protein (N) is a basic RNA binding protein that is highly conserved among MHV strains and plays a major role in the extent of neurovirulence. It also plays structural roles by both complexing with genome RNA to form the viral capsid and interacting with the viral membrane protein (M) during virion assembly. It has three conserved regions (I, II, and III) that are separated by two hypervariable regions. It is reported to associate with microtubules, suggesting a possible role for N in trafficking and axonal transport in neurons (Ref.: Cowley, TJ., et al. (2010). J. Virol. 84(4); 1752 1763; Miura, TA., et al. (2008). J. Virol. 82(2); 755-763).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality SegmentMQ200
      Applications
      ApplicationAnti-Murine Coronavirus MHV-JHM nucleocapsid, clone 1-16-1, Cat. No. MABF2751, mouse monoclonal antibody, detects Murine Coronavirus MHV-JHM nucleocapsid and is tested in Immunofluorescence, Immunohistochemistry, Immunoprecipitation & Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Western Blotting applications (Leibowitz, J.L., et al. (1987). Adv Exp Med Biol. 218; 321-31).

      Immunofluorescence Analysis: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Immunofluorescence applications (Nanda, S.K., et al. (2001). J Virol. 75(7):3352-62).

      Immunohistochemistry Applications: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Immunohistochemistry applications (Miura, T.A., et al. (2008). J Virol. 82(2):755-63; Navas, S., et al. (2001). J Virol. 75(5):2452-7).

      Immunoprecipitation Analysis: A representative lot detected Murine Coronavirus MHV-JHM nucleocapsid in Immunoprecipitation applications (Nanda, S.K., et al. (2004). Arch Virol. 149(1):93-111).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRobb/Lampert strain of MHV-JHM (live whole virus).
      EpitopeUnknown
      Clone1-16-1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1-16-1 is a mouse monoclonal antibody that detects nucleoprotein of Murine coronavirus (MHV-JHM.IA).
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteMurine Coronavirus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • N
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 49.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse delayed brain tumor (DBT) cell lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Murine Coronavirus MHV-JHM nucleocapsid in Mouse delayed brain tumor (DBT) cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABF2751-25UL 04065266791365