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AB10614 Anti-PSMA (CT) Antibody

Anti-PSMA (C-Terminal) Antibody detects level of PSMA (C-Terminal) & has been published & validated for use in WB.

Anti-PSMA (CT) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Tumor Markers Gene Symbol: PSMA1, MGC22853, MGC14751, PROS30, HC2, PROS-30, MGC1667, MGC21459, PSC2, MGC14542, MGC23915, NU, MGC14575 UniProt Number: P25786, P25787, P25788, P28066, P60900, O14818
AB10614
100 µg  
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Overview

Replacement Information
Replacement InformationMABN1035 is the recommended replacement for Discontinued catalog number AB10614

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB10614
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-PSMA (CT) Antibody
Alternate Names
  • Prostate Specific Membrane Antigen
Background InformationPSMA (prostate specific membrane antigen) is a type II, integral membrane glycoprotein, composed of a 19 amino acid intracellular domain containing the N-terminus, a 24 amino acid transmembrane region, and a 707 amino acid extracellular C-terminal domain. The PSMA gene has been cloned and sequenced, and has been localized to chromosome 11q. PSMA is highly expressed in prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and in prostate cancer. On the basis of its highly prostate-specific nature, PSMA has been used as a prostate cancer marker antigen and diagnostic target for prostate cancer detection. Recently, evidence of limited and specific endothelial PSMA expression has been discovered: the neovasculature of a wide variety of malignant neoplasms (lung, colon, breast, etc.) demonstrates PSMA expression. This finding suggests that PSMA may be an effective target for monoclonal antibody-based anti-neovasculature therapy. This may be particularly true for antibodies directed against epitopes within the C-terminal region of PSMA, based on the high degree of accessibility for antibody binding due to the extracellular location of this domain. PSMA has also been identified in the human nervous system. Based on activity, immunoreactivity, and mRNA sequence comparison with one form of NAALADase, a neuropeptide that may modulate glutaminergic transmission in the nervous system, PSMA was found to be indistinguishable from NAALADase derived from human cerebellar cell isolates. Therefore, the form of NAALADase also known as PSMA is expressed in brain, where it makes up a significant fraction of brain NAALADase activity.
References
Product Information
FormatPurified
PresentationThis polyclonal antibody is supplied in 400 μL of phosphate buffered saline (PBS), pH 7.4, containing 0.05% sodium azide (NaN3).
Applications
ApplicationAnti-PSMA (C-Terminal) Antibody detects level of PSMA (C-Terminal) & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blotting: 1-3 μg/mL
Biological Information
ImmunogenA synthetic peptide derived from the C-terminal region of human PSMA.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes PSMA (C-Terminal).
Species Reactivity
  • Human
Species Reactivity NoteHuman (positive controls: LNCaP FGC cells, prostate cancer tissues)
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.
Gene Symbol
  • PSMA1
  • MGC22853
  • MGC14751
  • PROS30
  • HC2
  • PROS-30
  • MGC1667
  • MGC21459
  • PSC2
  • MGC14542
  • MGC23915
  • NU
  • MGC14575
Purification MethodImmunoAffinity Purified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P60900 # The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
SIZE: 246 amino acids; 27399 Da
SUBUNIT: The proteasome is composed of at least 15 non identical subunits which form a highly ordered ring-shaped structure.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
SIMILARITY: SwissProt: P60900 ## Belongs to the peptidase T1A family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFreeze -20°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB10614 04053252332975

Documentation

Required Licenses

Title
PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

References

Reference overviewPub Med ID
Five different anti-prostate-specific membrane antigen (PSMA) antibodies confirm PSMA expression in tumor-associated neovasculature
Chang, S S, et al
Cancer Res, 59:3192-8 (1999)  1999

10397265 10397265
Isolation and characterization of monoclonal antibodies specific for the extracellular domain of prostate specific membrane antigen
Murphy, G P, et al
J Urol, 160:2396-401 (1998)  1998

9817391 9817391