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ABS1675 Anti-PXDN/VPO1 Antibody

This Anti-PXDN Antibody is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation for the detection of PXDN.

Anti-PXDN/VPO1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: ECM Proteins Gene Symbol: PXDN, COPOA, KIAA0230, MG50, PRG2, VPO, VPO1 UniProt Number: Q92626
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ABS1675
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, BWB, IH(P), IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1675
      DescriptionAnti-PXDN/VPO1 Antibody
      Alternate Names
      • Peroxidasin homolog
      • hsPxd01
      • Melanoma-associated antigen MG50
      • p53-responsive gene 2 protein
      • Vascular peroxidase 1
      Background InformationPeroxidasin homolog (EC 1.11.1.7; UniProt Q92626; also known as hsPxd01, Melanoma-associated antigen MG50, p53-responsive gene 2 protein, Vascular peroxidase 1) is encoded by the PXDN (also known as COPOA, KIAA0230, MG50, PRG2, VPO, VPO1) gene (Gene ID 7837) in human. Human peroxidasin 1 (hsPxd01) belong to the superfamily of heme peroxidases with important roles in innate immunity and hormone biosynthesis, members of which also include myeloperoxidase (MPO), eosinophil peroxidase (EPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO). Peroxidase 1 is a multidomain heme peroxidase that uses bromide as a cofactor in catalyzing the formation of sulfilimine bonds in collagen IV via release of hypohalous acids. Peroxidase 1 plays an essential role in tissue development and architecture by catalyzing sulfilimine bonds formation that confers critical structural reinforcement to collagen IV scaffolds. Human peroxidasin 1 is produced with a signal peptide sequence (a.a. 1-26), the remvoal of which yields the mature protein. In its N-terminal region, hsPxd01 contains four leucine-rich (LRR) repeats (a.a.87-180) flanked by two cysteine-rich domains, the LRRNT (a.a. 27-63) and LRRCT (a.a. 192-245), this is followed by four C2-type Ig-like domains, and a C-terminal von Willebrand factor type C domain (VWFC; a.a. 1413-1471) found in many secreted extracellular matrix proteins.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in 0.1 M Tris-glycine buffer containing 0.02% sodium azide.
      Quality SegmentMQ100
      Applications
      ApplicationThis Anti-PXDN Antibody is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation for the detection of PXDN.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:400 diluttion from a representative lot detected VPO1 in mouse lung tissue sections (Courtesy of Guangjie Cheng, M.D., Emory University, GA, U.S.A.).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated all the radiolabelled VPO1 from the culture supernatant, but not from cell lysates, 20 hrs after an initial 20-min [32S]-Met pulse labeling of HEK293 cells stably expressing transfected VPO1 (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).
      Western Blotting Analysis: A representative lots detected cellular VPO1 in total cell lysate and secreted VPO1 in concentrated culture medium following histone deacetylase inhibition by NaBu (Cat. No. 567430) treatment of HEK293 cells stably expressing exogenously transfected VPO1 (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).
      Western Blotting Analysis: A representative lot detected VPO1 in human, mouse, and bovine sera samples, as well as a time-dependent cellular VPO1 upregulation in lysates from LPS- or TNF-alpha-stimulated HUVECs (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a LRRNT domain sequence of human PXDN/VPO1.
      EpitopeLRRNT domain.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody was raised against an N-terminal region sequence present in both spliced isoforms of human VPO1/PXDN protein (UniProt Q92626), but not conserved in MPO, LPO, EPO and TPO (Cheng, G., et al. (2011). Free Radic. Biol. Med. 51(7):1445-1453).
      Species Reactivity
      • Human
      • Mouse
      • Bovine
      Species Reactivity NoteHuman, Mouse, Bovine.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PXDN
      • COPOA
      • KIAA0230
      • MG50
      • PRG2
      • VPO
      • VPO1
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight>165 kDa observed. 162.4/165.3 kDa (human isoform 1 mature/pro-form), 77.50/80.33 kDa (human isoform 2 mature/pro-form), 162.4/165.1 kDa (mouse mature/pro-form) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting Analysis in human plasma.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected VPO1 in 10 µg of human plasma.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      ABS1675 04055977351637