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AB3353 Anti-SUV39H1 Antibody

Use Anti-SUV39H1 Antibody (Rabbit Polyclonal Antibody) validated in ELISA, WB to detect SUV39H1 also known as KMT1A, Histone H3-K9 Methyltransferase 1.

Anti-SUV39H1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: SUV39H1, SUV39H, Su, KMT1A, MG44, EC 2.1.1.43 UniProt Number: O43463
AB3353
100 µg  
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Overview

Replacement Information
Replacement InformationMABE552 is the recommended replacement for Discontinued catalog number AB3353

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB3353
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-SUV39H1 Antibody
Alternate Names
  • Suppressor of Variegation 3-9 Homolog
  • KMT1A
  • Histone H3-K9 Methyltransferase 1
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in PBS containing 0.09% sodium azide.
Applications
ApplicationUse Anti-SUV39H1 Antibody (Rabbit Polyclonal Antibody) validated in ELISA, WB to detect SUV39H1 also known as KMT1A, Histone H3-K9 Methyltransferase 1.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern Blot: 1:100-1:500. Predicted molecular weight 47 kDa.

ELISA (direct): 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenGST fusion protein from the N-terminal of human SUV39H1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityReacts with SUV39H1.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression.
Gene Symbol
  • SUV39H1
  • SUV39H
  • Su
  • KMT1A
  • MG44
  • EC 2.1.1.43
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O43463 # Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length.


SIZE: 412 amino acids; 47907 Da

SUBUNIT: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR.

SUBCELLULAR LOCATION: Nucleus. Note=Associates with centromeric constitutive heterochromatin.

DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.

DOMAIN: SwissProt: O43463 Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.

PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.


SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. & Contains 1 chromo domain. & Contains 1 post-SET domain. & Contains 1 pre-SET domain. & Contains 1 SET domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
AB3353 04053252286506

Documentation

Anti-SUV39H1 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-SUV39H1 POLYCLONAL ANTIBODY - 2197122 2197122

References

Reference overviewPub Med ID
Histone methyltransferases, diet nutrients and tumour suppressors.
Huang, Shi
Nat. Rev. Cancer, 2: 469-76 (2002)  2002

12189389 12189389
Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase.
Wang, H, et al.
Mol. Cell, 8: 1207-17 (2001)  2001

Show Abstract 		11779497 11779497