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MABS1753-25UG Anti-TC PTPase (PTPN2) Antibody, clone 9E9.1

Anti-TC PTPase (PTPN2), clone 9E9.1, Cat. No. MABS1753, is a mouse monoclonal antibody that detects T-cell protein-tyrosine phosphatase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category: Signaling Gene Symbol: PTPN2, PTPT UniProt Number: P17706
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MABS1753-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1753-25UG
      DescriptionAnti-TC PTPase (PTPN2) Antibody, clone 9E9.1
      Alternate Names
      • Tyrosine-protein phosphatase non-receptor type 2
      • EC: 3.1.3.48
      • T-cell protein-tyrosine phosphatase
      • TCPTP
      Background InformationTyrosine-protein phosphatase non-receptor type 2 (UniProt: P17706; also known as EC: 3.1.3.48, T-cell protein-tyrosine phosphatase, TCPTP) is encoded by the PTPN2 (also known as PTPT) gene (Gene ID: 5771) in human. TCPTP is a non-receptor type tyrosine-specific phosphatase that dephosphorylates a variety of receptor protein tyrosine kinases. It is also reported to dephosphorylate non-receptor tyrosine kinases, such as JAK 1, 2, 3, Src kinases, STAT1, 3, 5, 5B, and 6. TCPTP is ubiquitously expressed and two isoforms have been reported. Isoform 1 is expressed in T-cells and in placenta and is targeted to the endoplasmic reticulum by its C-terminal hydrophobic region. Isoform 2 is reported to be the major isoform that predominantly localizes to chromatin and is able to shuttle between the nucleus and the cytoplasm and to plasma membrane to dephosphorylate receptors. It doing so it negatively regulates numerous signaling pathways and biological processes, including hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. It is also reported to play a multifaceted and important role in the development of the immune system. In T-cell receptor signaling, it functions through dephosphorylation of FYN and LCK to control T-cell differentiation and activation. It also dephosphorylates CSF1R and thereby negatively regulates its downstream signaling and macrophage differentiation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-TC PTPase (PTPN2), clone 9E9.1, Cat. No. MABS1753, is a mouse monoclonal antibody that detects T-cell protein-tyrosine phosphatase and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TC PTPase (PTPN2) in human liver, human tonsil, and human cervical cancer tissues.
      Western Blotting Analysis: 0.5 µg/mL from a representative lot detected TC PTPase (PTPN2) in 10 µg of  Jurkat cell lysate.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 79 amino acids from the C-terminal half of human T-cell protein-tyrosine phosphatase.
      Clone9E9.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9E9.1 detects human T-cell protein-tyrosine phosphatase. It targets an epitope within 79 amino acids from the C-terminal half.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PTPN2
      • PTPT
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~48 KDa observed; 48.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.
      Western Blotting Analysis: 0.5 µg/mL of this antibody detected TC PTPase (PTPN2) in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABS1753-25UG 04054839522208

      Documentation