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OP129 Anti-TRF2 Mouse mAb (4A794)

Anti-TRF2 Mouse mAb (4A794) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TTAGGG Repeat Binding Factor 2

OP129
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~66 kDa (doublet) TRF2 protein in HL60 cells.
Catalogue NumberOP129
Brand Family Calbiochem®
SynonymsAnti-TTAGGG Repeat Binding Factor 2
Application Data
Detection of human TRF2 by immunoprecipitation, followed by immunoblotting. Sample: Whole cell lysate from 1x106 HL-60 cells. Antibodies for IP: Anti-TRF2 Mouse mAb (4A794) (2 µg) (lane 1), control mouse IgG (lane 2), purified goat-anti-TRF2 (lane 3) or pre-immune goat IgG (lane 4). Primary antibody for IB: Anti-TRF2 Mouse mAb (4A794) (Cat. No. OP129) (2 µg/ml). Detection: chemiluminescence.
References
ReferencesKarlseder, J., et al. 1999. Science 283, 1321.
Van Steensel, B., et al. 1998. Cell 92, 401.
Broccoli, D., et al. 1997. Nature Genetics 17, 231.
Product Information
FormLiquid
FormulationIn PBS, 0.05% BSA.
Positive controlHL-60 cells or most human cell lines
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (2 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (2 µg/1 million cells)
Application CommentsThe antibody recognizes distinct doublet bands of approximate size 66 kD corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines. The antibody also gives nuclear staining of cells by immunocytochemistry.
Biological Information
Immunogena recombinant protein consisting of full-length human TRF2 fused to a His•Tag® sequence, expressed in baculovirus
ImmunogenHuman
Clone4A794
HostMouse
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
OP129 0

Documentation

Anti-TRF2 Mouse mAb (4A794) SDS

Title

Safety Data Sheet (SDS) 

Anti-TRF2 Mouse mAb (4A794) Certificates of Analysis

TitleLot Number
OP129

References

Reference overview
Karlseder, J., et al. 1999. Science 283, 1321.
Van Steensel, B., et al. 1998. Cell 92, 401.
Broccoli, D., et al. 1997. Nature Genetics 17, 231.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-September-2008 RFH
SynonymsAnti-TTAGGG Repeat Binding Factor 2
ApplicationImmunoblotting (2 µg/ml)
Immunocytochemistry (5 µg/ml)
Immunoprecipitation (2 µg/1 million cells)
Application Data
Detection of human TRF2 by immunoprecipitation, followed by immunoblotting. Sample: Whole cell lysate from 1x106 HL-60 cells. Antibodies for IP: Anti-TRF2 Mouse mAb (4A794) (2 µg) (lane 1), control mouse IgG (lane 2), purified goat-anti-TRF2 (lane 3) or pre-immune goat IgG (lane 4). Primary antibody for IB: Anti-TRF2 Mouse mAb (4A794) (Cat. No. OP129) (2 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~66 kDa huTRF2 protein.
BackgroundTRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of full-length human TRF2 fused to a His•Tag® sequence, expressed in baculovirus
Clone4A794
IsotypeIgG
Specieshuman
Positive controlHL-60 cells or most human cell lines
FormLiquid
FormulationIn PBS, 0.05% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThe antibody recognizes distinct doublet bands of approximate size 66 kD corresponding to the molecular size of huTRF2 by western blot analysis against lysates from most human cell lines. The antibody also gives nuclear staining of cells by immunocytochemistry.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKarlseder, J., et al. 1999. Science 283, 1321.
Van Steensel, B., et al. 1998. Cell 92, 401.
Broccoli, D., et al. 1997. Nature Genetics 17, 231.