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MABE1008 Anti-TRRAP Antibody, clone 2TRR-1B3

Anti-TRRAP Antibody, clone 2TRR-1B3 is an antibody against TRRAP for use in Western Blotting, Immunoprecipitation.

Anti-TRRAP Antibody, clone 2TRR-1B3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: TRRAP, PAF400 UniProt Number: Q9Y4A5
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MABE1008
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABE1008
      DescriptionAnti-TRRAP Antibody, clone 2TRR-1B3
      Alternate Names
      • Transformation/transcription domain-associated protein
      • 350/400 kDa PCAF-associated factor
      • PAF350/400
      • STAF40
      • TR-AP
      • Tra1 homolog
      Background InformationTransformation/transcription domain-associated protein (UniProt Q9Y4A5; also known as 350/400 kDa PCAF-associated factor, PAF350/400, STAF40, TR-AP, Tra1 homolog) is encoded by the TRRAP (also known as PAF400) gene (Gene ID 8295) in human. Transformation/transcription domain-associated protein (TRRAP) is a cofactor component shared by several histone acetyltransferase (HAT) complexes, including SAGA (Spt-Ada-Gcn5-acetyltransferase), PCAF (P300/CBP-associated factor), and Tip60. TRRAP is an essential component of the histone epigenetic modifying mechinery that plays an important role in regulating cell-cycle progression. Trrap gene-knockdown or protein downregulation causes cell and mouse lethality, impaired spindle checkpoint and differentiation.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG2aκ ascites without preservatives.
      Quality SegmentMQ100
      Applications
      ApplicationAnti-TRRAP Antibody, clone 2TRR-1B3 is an antibody against TRRAP for use in Western Blotting, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 10 µL from a representative lot immunoprecipitated TRRAP from 1 mL (4.5 mg/mL) of HeLa nuclear extract (Courtesy of Dr. Mustapha OULAD-ABDELGHANI, IGBMC, France).
      Immunoprecipitation Analysis: A representative lot immunodepleted TRRAP using nonhomologous end-joining/NHEJ-competent protein extracts prepared from human AAH-1 lymphoblastoid cells (Robert, F., et al. (2006). Mol. Cell. Biol. 26(2):402-412).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to a sequence from the TP53-interacting region of human TRRAP.
      EpitopeN-terminal to nuclear localization signal in the TP53-interacting region.
      Clone2TRR-1B3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThe immunogen sequence is present in both spliced isoforms of human TRRAP reported by UniProt (Q9Y4A5).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRRAP
      • PAF400
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~400 kDa observed. 437.6 kDa (isoform 1) and 434.4 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvalulated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TRRAP in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      MABE1008 04055977344141