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PC04 Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb

Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~185 kDa c-ErbB2/c-Neu protein in Sk-BR-3 cells.
      Catalogue NumberPC04
      Brand Family Calbiochem®
      SynonymsAnti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus
      References
      ReferencesDi Fiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Negative controlNormal human fibroblasts
      Positive controlSK-BR-3 cells
      PreservativeNone
      Applications
      Application ReferencesParaffin Sections King, B.L., et al. 1992. Am. J. Pathol 140, 23.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see application references)
      Application CommentsAntibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogena synthetic peptide (LARLLDIDETEYHAD) corresponding to amino acids 866-880 from the kinase domain of human c-Neu
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      PC04 0

      Documentation

      Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-c-ErbB2/c-Neu (Ab-1) (866-880) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC04

      References

      Reference overview
      Di Fiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      SynonymsAnti-HER2, Anti-c-Neu, Anti-Erythroblastosis Virus
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see application references)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~185 kDa native and denatured forms of c-ErbB2/c-Neu.
      BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification may be a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways, and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. Neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal growth factor Receptor) and is the second member of the EGF receptor family to be described.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (LARLLDIDETEYHAD) corresponding to amino acids 866-880 from the kinase domain of human c-Neu
      IsotypeIgG
      Specieshuman, rat
      Positive controlSK-BR-3 cells
      Negative controlNormal human fibroblasts
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual sample types.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDi Fiore, P.P., et al. 1987. Science 237, 178.
      Slamon, D.J., et al. 1987. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Application referencesParaffin Sections King, B.L., et al. 1992. Am. J. Pathol 140, 23.