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05-628 Anti-phospho-Bad (Ser155) Antibody, clone JBW101

Anti-phospho-Bad (Ser155) Antibody, clone JBW101 is an antibody against phospho-Bad (Ser155) for use in WB.

Anti-phospho-Bad (Ser155) Antibody, clone JBW101 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: BCL2 & Inhibition Gene Symbol: BAD, BBC2, BBC6, BCL2L8 UniProt Number: Q92934
05-628
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-628
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-Bad (Ser155) Antibody, clone JBW101
References
Product Information
FormatPurified
Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, 5mg/ml BSA before the addition of glycerol to 30%
Applications
ApplicationAnti-phospho-Bad (Ser155) Antibody, clone JBW101 is an antibody against phospho-Bad (Ser155) for use in WB.
Key Applications
  • Western Blotting
Biological Information
Immunogensynthetic peptide (C-YGRELRRM[pS]DEFEG) corresponding to amino acids 147-160 of mouse Bad
CloneJBW101
HostMouse
Specificityphosphorylated Bad
IsotypeIgG2b
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.
Gene Symbol
  • BAD
  • BBC2
  • BBC6
  • BCL2L8
Modifications
  • Phosphorylation
Purification MethodProtein G Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q92934 # Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
SIZE: 168 amino acids; 18392 Da
SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity).
SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
TISSUE SPECIFICITY: Expressed in a wide variety of tissues.
DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity).
SIMILARITY: Belongs to the Bcl-2 family.
Molecular Weight30kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on recombinant soluble murine Bad (Catalog #14-357) phosphorylated at Ser155 by PKA (Catalog #14-111)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
05-628 04053252287015