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17-198 Caspase 3 Activity Detection Kit

Caspase 3 Activity Detection Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Activity Assay
17-198
1 kit  Kit capacity: 130 assays
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsDetection Methods
ACTFluorescent
Description
Catalogue Number17-198
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCaspase 3 Activity Detection Kit
References
Product Information
Components
  • Caspase 3 Fluorometric Substrate (Cat.# 12-323)
  • Caspase 3, active (Cat.# 14-264)
Detection methodFluorescent
Applications
Key Applications
  • Activity Assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Gene Symbol
  • CASP3
  • apopain
  • CASP-3
  • procaspase3
  • CPP32B
  • SCA-1
  • CPP-32
  • Apopain
  • CPP32
  • Yama
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
SIZE: 277 amino acids; 31608 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated in a Protease Assay to hydrolyze Caspase 3 fluorometric substrate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Material PackageKit capacity: 130 assays
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
17-198 04053252404481

Related Products & Applications

Kit Component

Catalogue Number Description
12-323 Caspase 3 Fluorometric Substrate

Product Families

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Millipore’s Caspase Antibodies, proteins, and assays have been well validated and published. See below for a comprehensive list of our Caspase products, based on the expertise of Upstate & Chemicon.
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Categories

Life Science Research > Protein Detection and Quantification > Activity Assays > Caspase & Apoptosis Detection