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17-10527 EdU Cell Proliferation Assay (EdU-594)

The EdU-594 Cell Proliferation Assay is a superior alternative to conventional BrdU assays. The assay uses click chemistry to fluorescently detect the incorporation of EdU into newly synthesized DNA.

EdU Cell Proliferation Assay (EdU-594) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
17-10527
100 reactions  
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      Overview

      Replacement Information

      Key Spec Table

      Detection Methods
      Fluorescence
      Description
      Catalogue Number17-10527
      DescriptionEdU Cell Proliferation Assay (EdU-594)
      OverviewThe use of EdU (5-ethynyl-2’-deoxyuridine) as a thymidine nucleoside analog is a significant improvement compared to the classical BrdU and 3[H] thymidine cell proliferation assays. In contrast to BrdU assay kits, the EdU cell proliferation assays are not antibody based and do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU Cell Proliferation assays measure the incorporation of EdU into newly synthesized DNA with click chemistry for detection by fluorescence microscopy and flow cytometry applications.

      • Fast, simple workflow
      • No DNA denaturation
      • No antibody
      • Structurally preserves sample
      • Fast detection procedure (~30 minutes)
      • Compatible with flow cytometry and microscopy
      • Kits available with a variety of high resolution fluorescent readouts (EdU488, 555, 594, 647)
      Materials Required but Not DeliveredReagents:
      • Cells adherently grown on a coverslip
      • Phosphate-buffered saline (PBS, pH 7.2-7.6)
      • Appropriate cell culture medium
      • Fixation solution (3.7% formaldehyde in PBS)
      • Permeabilization solution (for example, 0.5% Triton™ X-100 in PBS)
      • Deionized water

      Equipment:
      • Reaction tubes (size depends on the volume of reaction cocktail needed)
      • 18 x 18 mm coverslips
      • Optional: 6-well microplate
      References
      Product Information
      Components
      • 5-Ethynyl-deoxyuridine (5-EdU) (Part No. CS219082): One 5 mg vial
      • 5/6-Sulforhodamine101-PEG3-Azide (10 mM) (Part No. CS219039): One 130 µL vial
      • Buffer additive (Part No. CS219074): Four 200 mg vials
      • DMSO (Part No. CS219084): Two 2 mL vials
      • 10X Reaction Buffer (Part No. CS219085): Four 2 mL vials
      • Catalyst solution (Part No. CS219038): Two 2 mL vials
      Detection methodFluorescence
      Quality SegmentMQ100
      Applications
      ApplicationThe EdU-594 Cell Proliferation Assay is a superior alternative to conventional BrdU assays. The assay uses click chemistry to fluorescently detect the incorporation of EdU into newly synthesized DNA.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain the kit at 2° to 8°C for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 reactions
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      17-10527 04055977175240