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14-828 HDAC4, active recombinant protein

Recominant human HDAC4 (a.a. 627-end) with N-terminal GST-tag.

HDAC4, active recombinant protein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Enzyme Assays UniProt Number: P56524
14-828
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      Overview

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      Key Spec Table

      Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
      WB, Enzyme AssaysNM_006037 Human P56524
      Description
      Catalogue Number14-828
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHDAC4, active recombinant protein
      OverviewRecominant human HDAC4 (a.a. 627-end) with N-terminal GST-tag
      Background InformationHistone deacetylation acts a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. HDAC4 is a histone deacetylase which deacetylates lysines on the core histones. HDAC4 accumulates in the nuclei of myotubes, suggesting involvement in muscle differentiation. HDAC4 does not bind DNA directly but interacts with the myocyte enhancer factors Mef2a, Mef2c, and Mef2d. HDAC4 also interacts with the breast cancer-associated transcriptional repressor PLU-1/JARID1B, the Nr2c1/TR2 nuclear receptor, as well as the 14-3-3 chaperone protein, in a phosphorylation dependent manner. It also forms part of a multiprotein complex with RbAp48 and HDAC3.
      References
      Product Information
      FormatPurified
      Presentation50 μg of recombinant protien in 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 20% glycerol. Frozen solution.
      Applications
      ApplicationRecominant human HDAC4 (a.a. 627-end) with N-terminal GST-tag.
      Key Applications
      • Western Blotting
      • Enzyme Assays
      Application NotesHDAC deacetylase assays
      Biological Information
      Concentration0.2 mg/ml
      PurityPurity >90% by SDS-PAGE and Coomassie blue staining.
      SourceExpressed by baculovirus in Sf9 insect cells.
      Specific Activity[Varies with lot. For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.] Lot R0801D0003: 2.7 U/mg, where one unit of HDAC4 activity is defined as 1 nmol of assay substrate (HDAC assay kit, catalog #17-356) deacetylated per minute at 37°C.
      Entrez Gene Number
      Gene Symbol
      • HD4
      • HDAC4
      Purification MethodPurified using glutathione-agarose. GST portion was cleaved by PreScission™ protease.
      UniProt Number
      Molecular Weight49 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceHDAC4 Deacetylase Assay: 2 µg of this lot of enzyme deacetylated 100 µM assay substrate using the HDAC Assay Kit, Catalog # 17-356
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in aliquots at -70°C. Avoid freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing cap.
      Packaging Information
      Material Size1 each
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      14-828 04053252409875

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes