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SCC148 MB49 Mouse Bladder Carcinoma Cell Line

MB49 mouse urothelial carcinoma cell line is widely used as an in vitro and in vivo model of bladder cancer.

MB49 Mouse Bladder Carcinoma Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture
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SCC148
≥1X10⁶ cells/vial  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      CULT
      Description
      Catalogue NumberSCC148
      DescriptionMB49 Mouse Bladder Carcinoma Cell Line
      OverviewMB49 cells are derived from C57BL/Icrf-a’ mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture (1). Transformed cells transplanted into syngeneic mice were shown to generate carcinomas (1). While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed (2). This abnormality is a frequent early event in human bladder cancer.

      A recent study indicates that MB49 cells recapitulate key features of sex differences in bladder tumor growth (3). MB49 implantation in mice resulted in significantly larger tumors in males than females. In the presence of dihydrotestosterone, MB49 cells exhibited enhanced proliferation in a dose-dependent manner. In contrast, MB49 cells were unresponsive to the pregnancy hormone, human chorionic gonadotrophin (hCG) (3).

      MB49 cells exhibit low to no expression of MHC-Class I and II molecules (4). However, upon exposure to IFN-, the expressions of MHC Class I and II are significantly upregulated (4).

      References:
      1. Summerhayes IC, Franks LM (1979) Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro. J Natl Cancer Inst 62(4): 1017 - 1023.
      2. Fabris VT, Lodilinsky C, Pampena MB, Belgorosky D, Lanari C, Eiján AM (2012) Cytogenetic characterization of the murine bladder cancer model MB49 and the derived invasive line MB49-I. Cancer Genet 205(4): 168 - 76.
      3. White-Gilbertson S, Davis M, Voelkel-Johnson C, Kasman LM (2016) Sex differences in the MB49 syngeneic, murine model of bladder cancer. Bladder (San Franc) 3(1): PMID: 26998503.
      4. Lattime EC, Gomelia LG, McCue PA (1992) Murine bladder carcinoma cells present antigen to BCG-specific CD4+ T-cells. Cancer Res 52(15): 4286 - 90.
      Alternate Names
      • MB-49
      References
      Product Information
      Quality SegmentMQ100
      Applications
      ApplicationMB49 mouse urothelial carcinoma cell line is widely used as an in vitro and in vivo model of bladder cancer.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      SCC148 04054839394805