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HCMPEX1-19K MILLIPLEX® Human Complement Expanded Panel 1

The MILLIPLEX® Human Complement Expanded Panel 1, using the Luminex® xMAP® technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples.

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HCMPEX1-19K

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Description
Catalogue NumberHCMPEX1-19K
ReplacesHCMP1MAG-19K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Complement Expanded Panel 1
OverviewThe MILLIPLEX® Human Complement Expanded Panel 1 is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, or culture supernatant samples: C2, C4b, C5, C5a, C9, Factor D (Adipsin), Factor I, Mannose-Binding Lectin (MBL), and Properdin.
Background InformationThe complement system consists of a large number of plasma proteins that assist, or complement, the ability of phagocytic cells and antibodies to clear pathogens. Although it is part of the innate immune system, not adaptable and unchanging over the course of a lifetime, it can be recruited and activated by the adaptive immune system. The system proteins and glycoproteins that are synthesized primarily by hepatocytes normally circulate as inactive pro-proteins. Once stimulated the end result of the cascade is a massive amplification of response and activation of the membrane attack complex, which forms a transmembrane pore causing osmotic lysis of target cells.
The complement system consists of three different simultaneous pathways. The classical pathway is stimulated by antigen-antibody complexes; the alternative pathway spontaneously activates on contact with pathogenic cell surfaces; the mannose-binding lectin pathway recognizes mannose sugars usually present only on pathogenic cell surfaces.
The complement system is thought to play a key role in many diseases with an immune component, such as asthma or sepsis, and in many autoimmune diseases, including systemic lupus erythematosus, inflammatory bowel disease, rheumatoid arthritis, and multiple sclerosis. It is also becoming increasingly associated with neurological diseases, such as Alzheimer’s Disease, and conditions such as spinal cord injuries.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: 20
  • Intra-assay: 10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality SegmentMQ200
Applications
ApplicationThe MILLIPLEX® Human Complement Expanded Panel 1, using the Luminex® xMAP® technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• Complement activation can occur in samples that are not handled properly. Proper sample collection, handling, and storage is necessary to achieve accurate assay results.
• Serum samples will typically produce higher complement values. EDTA Plasma is recommended to achieve more accurate complement values.
• This assay requires 25 µl of 1:1,000 diluted plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.
• To avoid complement activation in samples, which will affect the results, samples should be thawed as rapidly as possible, put on ice, and assayed immediately.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Complement C2
  • Complement C4b
  • Complement C5
  • Complement C5a
  • Mannose-Binding Lectin (MBL)
  • Complement Factor I
  • Complement C9
  • Properdin
  • Adipsin
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • C2: 68%
  • C4b: 97%
  • C5: 98%
  • C5a: 83%
  • C9: 94%
  • Factor D (Adipsin): 92%
  • Mannose-Binding Lectin (MBL): 93%
  • Factor I: 98%
  • Properdin: 101%
Physicochemical Information
Sensitivity
  • C2: 1.24 ng/mL
  • C4b: 0.46 ng/mL
  • C5: 0.86 ng/mL
  • C5a: 4.37 pg/mL
  • C9: 0.28 ng/mL
  • Factor D (Adipsin): 0.05 ng/mL
  • Mannose-Binding Lectin (MBL): 0.048 ng/mL
  • Factor I: 0.12 ng/mL
  • Properdin: 0.02 ng/mL
Standard Curve Range
  • C2: 0.82-600 ng/mL
  • C4b: 1.1-800 ng/mL
  • C5: 1.9-1,350 ng/mL
  • C5a: 2.7-2,000 pg/mL
  • C9: 1.0-750 ng/mL
  • Factor D (Adipsin): 0.08-60 ng/mL
  • Mannose Binding Lectin: 0.14-100 ng/mL
  • Factor I: 0.41-300 ng/mL
  • Properdin: 0.07-50 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
HCMPEX1-19K 04065267983400

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