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71537 Tag•off™ High Activity rEK

Increased cleavage rate of fusion proteins

Tag•off™ High Activity rEK MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Tag-Off High Activity Enterokinase

71537
  
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      Overview

      Replacement Information
      Description
      OverviewTag•off™ High Activity rEK is a highly purified preparation of the catalytic subunit of recombinant human enterokinase that recognizes the identical cleavage site as the native enzyme, AspAspAspAspLys↓ (Gasparian 2003). The Tag•off High Activity rEK substrate binding site (ArgArgArgLys) differs from the bovine enterokinase site (LysArgArgLys) by a single amino acid, which is believed to account for its higher substrate affinity and the resulting higher specific activity. The preparation has been functionally tested for activity with thioredoxin/human epidermal growth factor and thioredoxin/ human IL-13 fusion proteins (Gasparian). The Tag•off High Activity rEK as a highly purified (99% by Coomassie staining) enzyme solution and includes rEK 1X Dilution/Storage Buffer, 10X Cleavage Buffer, and a Cleavage Control Protein.

      This product has been discontinued.
      Catalogue Number71537
      Brand Family Novagen®
      SynonymsTag-Off High Activity Enterokinase
      References
      ReferencesGasparian, M.E., et al. 2003. Protein Expr. and Purif. 31, 133.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme needed to cleave 50 µg of fusion protein in 16 hours at 23°C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, and 2 mM CaCl₂, pH 7.4.
      •50 UTag•Off™ High-activity rEK
      •10 µgCleavage Control Protein
      •2 ml1X rEK Dilution/Storage Buffer
      •1 ml10X rEK Cleavage Buffer
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Catalogue Number GTIN
      71537 0

      Documentation

      Tag•off™ High Activity rEK Certificates of Analysis

      TitleLot Number
      71537

      References

      Reference overview
      Gasparian, M.E., et al. 2003. Protein Expr. and Purif. 31, 133.

      User Protocols

      Title
      TB458 Tag•off™ High Activity rEK