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662087 UCH-L1, His•Tag® , Mouse, Recombinant, E. coli

UCH-L1, His•Tag® , Mouse, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ubiquitin C-terminal Hydrolase L1, Ubiquitin thioesterase L1

662087
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





Full-length, recombinant, mouse UCHL-1 fused at the N-terminus to His•Tag® sequence and expressed in E. coli. UCH-L1 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. The expression of UCH-L1 is highly specific to cells of the neuroendocrine system and their tumors. UCH-L1 variants (Ile93Met) have been linked to Parkinson disease and may participate in processes critical for neuronal health. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
Catalogue Number662087
Brand Family Calbiochem®
SynonymsUbiquitin C-terminal Hydrolase L1, Ubiquitin thioesterase L1
References
ReferencesKurihara, LJ., et al. 2001. Hum. Mol. Genet. 10, 1963.
Johnston, S.C., et al. 1999. EMBO. J. 18, 3877.
Saigoh, K., et al. 1999. Nat. Genet. 23, 47.
Dang, L.C. et al. 1998. Biochem. 37, 1868.
Leroy, E., et al.1998. DNA. Res. 5, 397.
Day, I.N., et al. 1990. Biochem J. 268, 521.
Mayer, A.N., and Wilkinson, K.D. 1989. Biochem. 28, 166.
Pickart, C.M., et al. 1985. J. Biol. Chem. 260, 7903.
Product Information
FormulationIn 50 mM HEPES, 2 mM DTT, pH 8.0.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Catalogue Number GTIN
662087 0

Documentation

UCH-L1, His•Tag® , Mouse, Recombinant, E. coli Certificates of Analysis

TitleLot Number
662087

References

Reference overview
Kurihara, LJ., et al. 2001. Hum. Mol. Genet. 10, 1963.
Johnston, S.C., et al. 1999. EMBO. J. 18, 3877.
Saigoh, K., et al. 1999. Nat. Genet. 23, 47.
Dang, L.C. et al. 1998. Biochem. 37, 1868.
Leroy, E., et al.1998. DNA. Res. 5, 397.
Day, I.N., et al. 1990. Biochem J. 268, 521.
Mayer, A.N., and Wilkinson, K.D. 1989. Biochem. 28, 166.
Pickart, C.M., et al. 1985. J. Biol. Chem. 260, 7903.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-July-2009 JSW
SynonymsUbiquitin C-terminal Hydrolase L1, Ubiquitin thioesterase L1
DescriptionFull-length, recombinant, mouse UCHL-1 fused at the N-terminus to His•Tag® sequence and expressed in E. coli. UCH-L1 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. The expression of UCH-L1 is highly specific to cells of the neuroendocrine system and their tumors. UCH-L1 variants (Ile93Met) have been linked to Parkinson disease and may participate in processes critical for neuronal health. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
FormulationIn 50 mM HEPES, 2 mM DTT, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Typical enzyme concentration required to support in vitro substrate hydrolysis is 10-50 nM; pre-incubation with 10 mM DTT for 15 min is recommended to achieve maximum activity.
Purity≥95% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesKurihara, LJ., et al. 2001. Hum. Mol. Genet. 10, 1963.
Johnston, S.C., et al. 1999. EMBO. J. 18, 3877.
Saigoh, K., et al. 1999. Nat. Genet. 23, 47.
Dang, L.C. et al. 1998. Biochem. 37, 1868.
Leroy, E., et al.1998. DNA. Res. 5, 397.
Day, I.N., et al. 1990. Biochem J. 268, 521.
Mayer, A.N., and Wilkinson, K.D. 1989. Biochem. 28, 166.
Pickart, C.M., et al. 1985. J. Biol. Chem. 260, 7903.