Protocol- 46-667MAG
- Document Type:
- Protocols
- Product Catalog Number:
- 46-667MAG
- Product Catalog Name:
- MILLIPLEX MAP GAPDH Total Magnetic Bead MAPmate™ - Cell Signaling Single Plex or Multiplex Assay
46-667mag
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The Power of Biomarker Analysis
- Document Type:
- Brochure
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
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TNF-like weak inducer of apoptosis (TWEAK) promotes glioblastoma cell chemotaxis via Lyn activation.
- The long-term survival of patients with glioblastoma (GB) is compromised by the proclivity for local invasion into the surrounding normal brain, escaping surgical resection and contributing to therapeutic resistance. Tumor necrosis factor-like weak inducer of apoptosis (TWEAK), a member of the tumor necrosis factor superfamily, can stimulate glioma cell invasion via binding to fibroblast growth factor-inducible 14 (Fn14) and subsequent activation of the Rho GTPase family member Rac1. Here, we demonstrate that TWEAK acts as a chemotactic factor for glioma cells, a potential process for driving cell invasion into the surrounding brain tissue. TWEAK exposure induced the activation of Src-family kinases (SFK), and pharmacologic suppression of SFK activity inhibited TWEAK-induced chemotactic migration. We employed a multiplexed Luminex assay and identified Lyn as a candidate SFK activated by TWEAK. Depletion of Lyn suppressed TWEAK-induced chemotaxis and Rac1 activity. Furthermore, Lyn gene expression levels increase with primary glioma tumor grade and inversely correlate with patient survival. These results show that TWEAK-induced glioma cell chemotaxis is dependent upon Lyn kinase function, and thus, provides opportunities for therapeutic targeting of this deadly disease.
- Document Type:
- Reference
- Product Catalog Number:
- Multiple
- Product Catalog Name:
- Multiple
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96 well Template 2 Up
- Document Type:
- Technical Information
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96 well Template Single
- Document Type:
- Technical Information
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Decreased VEGFR2 expression and increased phosphorylated Akt1 in the prefrontal cortex of individuals with schizophrenia
- Document Type:
- Reference
- Product Catalog Number:
- Multiple
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Phospho Akt (Ser473) Assay Buffer 2 Magnetic Bead MAPmate¿
- Document Type:
- Protocols
- Product Catalog Number:
- 46-677MAG