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AB5154-50UL Anti-Cav2.2 Antibody

This Anti-Cav2.2 Antibody is validated for use in IC, IP, WB for the detection of Cav2.2.

Anti-Cav2.2 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters, Neuroinflammation & Pain Gene Symbol: CACNA1B, BIII, CACH5, Cav2.2, CACNN, CACNL1A5 UniProt Number: Q00975
AB5154-50UL
50 µL  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RICC, IP, WB, Immunohistochemistry (Tissue)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5154-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cav2.2 Antibody
      Alternate Names
      • Voltage Gated Calcium Channel (N-type) alpha 1B Subunit
      • Cacna1b
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder in phosphate buffered saline, pH 7.4, containing 5% sucrose and 0.025% sodium azide). The stock solution of the antigen can be made up using 200 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality SegmentMQ100
      Applications
      ApplicationThis Anti-Cav2.2 Antibody is validated for use in IC, IP, WB for the detection of Cav2.2.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Tissue)
      Application NotesIn preparing full-length protein samples for Western blot or immunoprecipitation, the procedures should be performed at 4°C, with the following protease inhibitor mixture: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

      Western blot: 1:100-1:200 using ECL on rat brain membranes.

      Immunohisto/cytochemistry on rat brain sections.

      Immunoprecipitation

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide from amino acids 851-867 of rat Cav2.2 (Accession Q02294).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Cav2.2 (alpha 1B subunit (both LMW and HMW forms) of voltage-gated calcium channel, Cacna1b). Does not cross react with any other calcium channel antigens tested so far.

      SPECIES REACTIVITIES: Rat. It is expected that the antibody will also work on mouse due to sequence homology (15/17). Other species have not been tested.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryVoltage-dependent Ca(2+) channels are multisubunit complexes found in the membrane of many excitable cells that regulate calcium entry (see MIM 601011). N-type calcium channels, which control neurotransmitter release from neurons, are dihydropyridine-insensitive and omega-conotoxin-sensitive. The alpha-1 subunit forms the pore through which calcium enters the cell, and is encoded by a family of at least 5 genes.[supplied by OMIM]
      Gene Symbol
      • CACNA1B
      • BIII
      • CACH5
      • Cav2.2
      • CACNN
      • CACNL1A5
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q00975 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the high-voltage activated (HVA) group and are blocked by omega-conotoxin-GVIA (omega-CTx-GVIA) and by omega-agatoxin- IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.
      SIZE: 2339 amino acids; 262496 Da
      SUBUNIT: Multisubunit complex consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with RIMS1 and RIMBP2 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Isoform Alpha-1b-1 and isoform Alpha-1b-2 are expressed in the central nervous system, but not in skeletal muscle or aorta.
      DOMAIN: SwissProt: Q00975 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
      PTM: Phosphorylated in vitro by CaM-kinase II, PKA, PKC and CGPK (By similarity).
      SIMILARITY: Belongs to the calcium channel alpha-1 subunit family. & Contains 1 EF-hand domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at 2-8°C in undiluted aliquots for up to 2 weeks. For longer periods, it is recommended to add glycerol (ACS grade or better) at a ratio of 1:1 and store the antibody at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles..
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Número de referencia GTIN
      AB5154-50UL 04053252735813

      Documentation

      Anti-Cav2.2 Antibody Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Cav2.2 Antibody Certificados de análisis

      CargoNúmero de lote
      RABBIT ANTI-Cav2.2 - 3244427 3244427
      RABBIT ANTI-Cav2.2 - 3317852 3317852
      RABBIT ANTI-Cav2.2 - 3448862 3448862
      RABBIT ANTI-Cav2.2 -2625891 2625891
      RABBIT ANTI-Cav2.2 -2734774 2734774
      RABBIT ANTI-Cav2.2 AFFINITY PURIFIED 2830901
      RABBIT ANTI-Cav2.2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3003455
      RABBIT ANTI-Cav2.2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3127913
      RABBIT ANTI-Cav2.2 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 3168319 3168319
      RABBIT ANTI-Cav2.2 AFFINITY PURIFIED POLYCLONAL ANTIBODY- 2827678 2827678
      Show more

      Ficha técnica

      Cargo
      RABBIT ANTI-Cav2.2 AFFINITY PURIFIED POLYCLONAL ANTIBODY

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies