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05-1524 Anti-Nuclear RNA export factor 1 Antibody, clone 53H8

Anti-Nuclear RNA export factor 1 Antibody, clone 53H8 is a mouse monoclonal antibody for detection of Nuclear RNA export factor 1 also known as Tip-associated protein, mRNA export factor TAP, has been validated in WB, IP, ICC.

Anti-Nuclear RNA export factor 1 Antibody, clone 53H8: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: NXF1, MEX67, Mex67, TAP UniProt Number: Q9UBU9
05-1524
100 µg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1524
      DescriptionAnti-Nuclear RNA export factor 1 Antibody, clone 53H8
      Alternate Names
      • Tip-associated protein
      • Tip-associating protein
      • mRNA export factor TAP
      • nuclear RNA export factor 1
      • tip associating protein
      Background InformationNuclear RNA export factor 1 (also known as Tap protein) is encoded by the NXF1 gene. The protein expressed by this gene is one member of a family of nuclear RNA export factor gene. The Nuclear RNA export factor 1 protein is involved in export of mRNA species bearing retroviral constitutive transport elements (CTE) from the nucleus as well as in the export of mRNA from the nucleus to the cytoplasm. This protein is localized predominantly in the nucleoplasm at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. However, this protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A) RNA. This protein is present in the nuclear Y14 complex and has been shown to interact with Aly/REF.
      References
      Product Information
      FormatPurified
      Control
      • SW-480 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Nuclear RNA export factor 1 Antibody, clone 53H8 is a mouse monoclonal antibody for detection of Nuclear RNA export factor 1 also known as Tip-associated protein, mRNA export factor TAP, has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Biological Information
      ImmunogenRecombinant protein corresponding to human nuclear RNA export factor 1.
      EpitopeUnknown
      Clone53H8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Nuclear RNA export factor 1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
      Gene Symbol
      • NXF1
      • MEX67
      • Mex67
      • TAP
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUINCTION: Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with THOC4 and THOC5.

      SUBUNIT STRUCTURE: Heterodimer with NXT1. Interacts (via NTF2 domain) with NXT1. The formation of NXF1/NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export. Interacts with NXT2, E1B-AP5, RAE1, THOC4 and with several nucleoporins. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, RENT1, RENT2, RENT3A, RENT3B, THOC4 and/or REFBP2. Found in a mRNA complex with RENT3A and RENT3B. Interacts with FYTTD1/UIF, EIF4A3, NUPL2 and THOC5. Interacts with Saimiriine herpesvirus 2 TIP protein.

      SUBCELLULAR LOCATION: Nucleus › nucleoplasm. Nucleus speckle. Cytoplasm. Note: Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA.

      TISSUE SPECIFICTY: Expressed ubiquitously.

      DOMAIN: The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.

      The nucleoporin binding domain consists of a NTF2 domain (also called NTF2-like domain) and a TAP-C domain (also called UBA-like domain). It has 2 nucleoporin-FG-repeats binding sites (one in the NTF2 and the other in the TAP-C domain) which contribute to nucleoporin association and act synergistically to export cellular mRNAs.

      The NTF2 domain is functional only in the presence of NXT1 and is essential for the export of mRNA from the nucleus.

      The TAP-C domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the TAP-C domain has a critical role in the interaction with nucleoporins.

      The leucine-rich repeats are essential for the export of mRNA from the nucleus.

      MISCELLANEOUS: The RNA-binding domain is a non-canonical RNP-type domain.

      SEQUENCE SIMILARITIES: Belongs to the NXF family.

      Contains 4 LRR (leucine-rich) repeats.

      Contains 1 NTF2 domain.

      Contains 1 RRM (RNA recognition motif) domain.

      Contains 1 TAP-C domain.
      Molecular Weight ~ 70 kDa Observed. Uncharacterized band is seen at ~17 kDa in variety of cell types tested (K562, HepG2, Hela Nuclear Extract).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in SW-480 cell lysate.

      Western Blot Analysis: 2 µg/ml of this antibody detected TAP on 10 µg of SW-480 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade ITEM Number
      Número de referencia GTIN
      05-1524 04053252589744

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies