Millipore Sigma Vibrant Logo
Atención: Nos hemos mudado. Los productos Merck Millipore ya no pueden adquirirse en MerckMillipore.comMás información

DR1041 Anti-TIP60 Rabbit pAb

This Anti-TIP60 Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of TIP60.

Anti-TIP60 Rabbit pAb: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-HIV-1 Tat interactive protein, Anti-cPLA2-interaction protein

View Products on Sigmaaldrich.com
DR1041
  
Purchase on Sigma-Aldrich

Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
OverviewRecognizes the ~60 kDa TIP60 protein in Saos-2 cells.
Catalogue NumberDR1041
Brand Family Calbiochem®
SynonymsAnti-HIV-1 Tat interactive protein, Anti-cPLA2-interaction protein
Application Data
Lane 1: Detection of human TIP60 by immunoblotting. Sample: Whole cell lysate (25 µg) from Saos-2 cells. Primary antibody: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:3000). Detection: chemiluminescence. Lanes 2 and 3: Detection of TIP60 by immunoprecipitation. Samples: Whole cell lysate (500 µg) from Saos-2 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:100). Conditions for immunoblotting: same as lane 1.
References
ReferencesMurr, R., et al. 2006. Nat. Cell Biol. 8, 91.
Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
Tang, Y., et al. 2006. Mol. Cell 24, 827.
Frank, S.R., et al. 2003. EMBO Rep. 6, 575.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlSaos-2 cells
Preservative≤0.1% sodium azide
Quality SegmentMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:3000)
Immunoprecipitation (1:100)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human TIP60 protein
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Número de referencia GTIN
DR1041 0

Documentation

Anti-TIP60 Rabbit pAb Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Anti-TIP60 Rabbit pAb Certificados de análisis

CargoNúmero de lote
DR1041

Referencias bibliográficas

Visión general referencias
Murr, R., et al. 2006. Nat. Cell Biol. 8, 91.
Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
Tang, Y., et al. 2006. Mol. Cell 24, 827.
Frank, S.R., et al. 2003. EMBO Rep. 6, 575.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-August-2007 RFH
SynonymsAnti-HIV-1 Tat interactive protein, Anti-cPLA2-interaction protein
ApplicationImmunoblotting (1:3000)
Immunoprecipitation (1:100)
Application Data
Lane 1: Detection of human TIP60 by immunoblotting. Sample: Whole cell lysate (25 µg) from Saos-2 cells. Primary antibody: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:3000). Detection: chemiluminescence. Lanes 2 and 3: Detection of TIP60 by immunoprecipitation. Samples: Whole cell lysate (500 µg) from Saos-2 cells (lane 2) and negative control sample (lane 3). Antibody for immunoprecipitation: Anti-TIP60 Rabbit pAb (Cat. No. DR1041) (1:100). Conditions for immunoblotting: same as lane 1.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~60 kDa TIP60 protein.
BackgroundTIP60 (accession no. Q92993) is a 60 kDa protein that is a member of the MYST family, which is a distinct class of histone acetyl-transferases (HAT). TIP60 was initially identified as a protein that interacts with the HIV protein, Tat, and shares no homology with the HATs, CBP/p300, or PCAF. TIP60 was recently shown to modulate DNA repair and p53 function. TIP60 also directly acetylates γ-H2AX and, together with the cofactor, TRRAP, binds to the chromatin surrounding sites of DNA double-stranded breaks in vivo. Furthermore, TIP60 associates with MYC and recruits it to the chromatin, which results in MYC-induced actetylation of histone H4. TIP60 directly associates with p53 and specifically acetylates it at K120, modulating the decision between cell cycle arrest and apoptosis. TIP60 acts as a transcription coactivator of p53 that enhances the activation of p21 and puma, leading to cell growth arrest and apoptosis. K120 actetylation is essential for p53-mediated puma activation and apoptosis, but is dispensable for both p53-mediated growth arrest, through p21 activation, and p53/MDM2 interaction. K120 is not acetylated by p300 and its actetylation by TIP60 does not affect the stability or DNA binding activity of p53.
HostRabbit
Immunogenrecombinant, human TIP60 protein
IsotypeIgG
Specieshuman
Positive controlSaos-2 cells
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMurr, R., et al. 2006. Nat. Cell Biol. 8, 91.
Sykes, S.M., et al. 2006. Mol. Cell 24, 841.
Tang, Y., et al. 2006. Mol. Cell 24, 827.
Frank, S.R., et al. 2003. EMBO Rep. 6, 575.