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AB1441 Anti-Tumor Necrosis Factor-α Antibody

AB1441
100 µg  
Purchase on Sigma-Aldrich

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Descripción

Replacement Information
Replacement Information04-1114 is the recommended replacement for Discontinued catalog number AB1441

Ofertas especiales

Tabla espec. clave

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB1441
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Tumor Necrosis Factor-α Antibody
Alternate Names
  • TNF-alpha
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Purified by HPLC. Liquid in Tris buffered saline (sterile filtered 0.2μm). No preservatives.
Applications
ApplicationAnti-Tumor Necrosis Factor-α Antibody detects level of Tumor Necrosis Factor-α & has been published & validated for use in ELISA & WB.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern blot: 1:100-1:1,000. Antibody will recognize a band at 17 kDa corresponding to TNFalpha.

ELISA: 1:100-1:1,000.

Final working dilutions must be determined by end user.
Biological Information
ImmunogenRecombinant human TNFalpha.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityReacts with human TNF-alpha.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Gene Symbol
  • TNF
  • TNFSF2
  • Cachectin
  • cachectin
  • DIF
  • TNF-alpha
  • OTTHUMP00000037669
  • TNFA
  • TNF-a
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
SIZE: 233 amino acids; 25644 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Número de referencia GTIN
AB1441 04053252361685

Documentation

Licencias necesarias

Título
PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

Anti-Tumor Necrosis Factor-α Antibody Certificados de análisis

CargoNúmero de lote
RABBIT ANTI-HUMAN TUMOR NECROSIS FACTOR-ALPHA (TNF#945;) POLYCLONAL ANTIBODY - 2117121 2117121
RABBIT ANTI-HUMAN TUMOR NECROSIS FACTOR-ALPHA (TNFa) POLYCLONAL ANTIBODY - 2197119 2197119
RABBIT ANTI-HUMAN TUMOR NECROSIS FACTOR-ALPHA (TNFα) POLYCLONAL ANTIBODY - 2436295 2436295
RABBIT ANTI-HUMAN TUMOR NECROSIS FACTOR-ALPHA (TNFα) POLYCLONAL ANTIBODY - 2445840 2445840

Referencias bibliográficas

Visión general referenciasPub Med ID
Sodium Pyruvate Modulates Cell Death Pathways in HaCaT Keratinocytes Exposed to Half-Mustard Gas.
Paromov V, Brannon M, Kumari S, Samala M, Qui M, Smith M, Stone W
Int J Toxicol  2010

Mostrar resumen
21300769 21300769
Inflammatory cytokine levels are influenced by interactions between THP-1 monocytic, U-373 MG astrocytic, and SH-SY5Y neuronal cell lines of human origin.
Klegeris, A and McGeer, P L
Neurosci. Lett., 313: 41-4 (2001)  2001

Mostrar resumen
11684335 11684335
TNF-alpha messenger RNA and protein expression in the uteroplacental unit of mice with pregnancy loss.
Gorivodsky, M, et al.
J. Immunol., 160: 4280-8 (1998)  1998

Mostrar resumen
9574530 9574530

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Familias de productos

Categorías

Life Science Research > Antibodies and Assays > Primary Antibodies