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OP29 Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)

This Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) is validated for use in FC, Frozen Sections, Gel Shift, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-3) (Mutant).

Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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OP29
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Descripción

Replacement Information

Tabla espec. clave

Species ReactivityHostAntibody Type
B, Ch, Ht, H, M, RMMonoclonal Antibody

Precios y disponibilidad

Número de referencia DisponiblidadEmbalaje Cant./Env. Precio Cantidad
OP29-100UG
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Disponibilidad a confirmar Disponibilidad a confirmar
En existencia 
Suspendido
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Debe confirmarse disponibilidad
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      El resto: se avisará
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      		 Ampolla de plást. 100 μg
      Recuperando precio...
      No pudo obtenerse el precio
      La cantidad mínima tiene que ser múltiplo de
      Maximum Quantity is
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      OP29-200UG
      Comprobando disponibilidad...
      —
      Disponibilidad a confirmar Disponibilidad a confirmar
      En existencia 
      Suspendido
      Cantidades limitadas disponibles
      Debe confirmarse disponibilidad
        El resto: se avisará
          El resto: se avisará
          Se avisará
          Póngase en contacto con el Servicio de Atención al Cliente
          Contact Customer Service

          		 Ampolla de plást. 200 μg
          Recuperando precio...
          No pudo obtenerse el precio
          La cantidad mínima tiene que ser múltiplo de
          Maximum Quantity is
          Al finalizar el pedido Más información
          Ahorró ()
           
          —
          Solicitar precio
          		 —
          Description
          OverviewRecognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.
          Catalogue NumberOP29
          Brand Family Calbiochem®
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlSK-OV-3 cells
          Positive controlA431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
          Preservative≤0.1% sodium azide
          Quality SegmentMQ100
          Applications
          Application ReferencesParaffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.
          Key Applications Flow Cytometry
          Frozen Sections
          Gel Shift
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Application NotesFlow Cytometry (1-20 µg/ml)
          Frozen Sections (10 µg/ml)
          Gel Shift (see comments)
          Immunoblotting (5 µg/ml)
          Immunofluorescence (1-20 µg/ml, see application references)
          Immunoprecipitation (1 µg per sample)
          Paraffin Sections (see application references)
          Application CommentsUnder non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
          ImmunogenHuman
          Epitopewithin amino acids 213-217
          ClonePAb240
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Bovine
          • Chicken
          • Hamster
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade ITEM Number
          Número de referencia GTIN
          OP29-100UG 07790788053888
          OP29-200UG 04055977208429

          Documentation

          Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Ficha datos de seguridad (MSDS)

          Título

          Ficha técnica de seguridad del material (MSDS) 

          Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) Certificados de análisis

          CargoNúmero de lote
          OP29

          Referencias bibliográficas

          Visión general referencias
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.

          Folleto

          Cargo
          Caspases and other Apoptosis Related Tools Brochure
          Ficha técnica

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2007 RFH
          ApplicationFlow Cytometry (1-20 µg/ml)
          Frozen Sections (10 µg/ml)
          Gel Shift (see comments)
          Immunoblotting (5 µg/ml)
          Immunofluorescence (1-20 µg/ml, see application references)
          Immunoprecipitation (1 µg per sample)
          Paraffin Sections (see application references)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions. Recognizes both the mutant and the wild-type p53 protein under denaturing conditions.
          BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
          HostMouse
          Immunogen speciesHuman
          Immunogena recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
          Epitopewithin amino acids 213-217
          ClonePAb240
          IsotypeIgG₁
          Speciesnot Xenopus, bovine, chicken, hamster, human, mouse, rat
          Positive controlA431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
          Negative controlSK-OV-3 cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsUnder non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
          Application referencesParaffin Sections Pezzella, F., et al. 1994. J. Clin. Pathol. 47, 592. Original Clone Gannon, J.V., et al. 1990. EMBO J. 9, 1595. Epitope Identification Stephen, C.W. and Lane, D.P., 1992. J. Mol. Biol. 225, 1. Immunofluorescence Bernardi, R., et al. 2004. Nat. Cell Biol. 6, 665.

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          Categorías

          Life Science Research > Antibodies and Assays > Primary Antibodies