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233903 Coelenterazine-h, Synthetic - CAS 50909-86-9 - Calbiochem

A highly sensitive and a specific intracellular luminophore that is useful for measuring changes in levels of superoxide and Ca2+ in cells transfected with apoaequorin cDNA.

Coelenterazine-h, Synthetic - CAS 50909-86-9 - Calbiochem: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: CLZ-h, 2-Benzyl-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one

233903
  
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Descripción

Replacement Information

Tabla espec. clave

CAS #Empirical Formula
50909-86-9C₂₆H₂₁N₃O₂
Description
OverviewA highly sensitive and a specific intracellular luminophore that is useful for measuring changes in levels of superoxide and Ca2+ in cells transfected with apoaequorin cDNA. Exhibits nearly 16-fold greater luminescence intensity (emission maximum ~466 nm; half-total time of 0.6 - 1.2 sec) as compared to the native Coelenterazine (Cat. No. 233900).
Catalogue Number233903
Brand Family Calbiochem®
SynonymsCLZ-h, 2-Benzyl-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
References
ReferencesNebl, T., and Fisher. P.R. 1997. J. Cell. Sci. 110, 2845.
Shimomura, O. 1997. Biochem. J. 326, 297.
Sanchez-Bueno, A., et al. 1996. Int. J. Biochem. Cell Biol. 28, 1045.
Knight, M.R., et al. 1993. J. Cell. Biol. 121, 83.
Shimomura, O., et al. 1993. Biochem. J. 296, 549.
Shimomura, O., et al. 1993. Cell Calcium 14, 373.
Shimomura, O., et al. 1989. Biochem. J. 261, 913.
Product Information
CAS number50909-86-9
ATP CompetitiveN
FormYellow brown solid
Hill FormulaC₂₆H₂₁N₃O₂
Chemical formulaC₂₆H₂₁N₃O₂
ReversibleN
Structure formula ImageStructure formula Image
Applications
Biological Information
Primary TargetMeasuring changes in levels of superoxide and Ca2+ in cells transfected with apoaequorin cDNA.
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot, purge with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade ITEM Number
Número de referencia GTIN
233903 0

Documentation

Coelenterazine-h, Synthetic - CAS 50909-86-9 - Calbiochem Ficha datos de seguridad (MSDS)

Título

Ficha técnica de seguridad del material (MSDS) 

Coelenterazine-h, Synthetic - CAS 50909-86-9 - Calbiochem Certificados de análisis

CargoNúmero de lote
233903

Referencias bibliográficas

Visión general referencias
Nebl, T., and Fisher. P.R. 1997. J. Cell. Sci. 110, 2845.
Shimomura, O. 1997. Biochem. J. 326, 297.
Sanchez-Bueno, A., et al. 1996. Int. J. Biochem. Cell Biol. 28, 1045.
Knight, M.R., et al. 1993. J. Cell. Biol. 121, 83.
Shimomura, O., et al. 1993. Biochem. J. 296, 549.
Shimomura, O., et al. 1993. Cell Calcium 14, 373.
Shimomura, O., et al. 1989. Biochem. J. 261, 913.
Ficha técnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-April-2011 RFH
SynonymsCLZ-h, 2-Benzyl-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
DescriptionA membrane-permeable, highly sensitive, specific, intracellular luminophore that is useful for measuring changes in Ca2+. For the measurement of Ca2+ levels coelenterazine is used in cells that have been transfected with apoaequorin cDNA. In this system, coelenterazine is required for the regeneration of aequorin, a protein that emits light in the presence of calcium, from apoaequorin produced in cells. The luminescence intensity appears to be directly proportional to the Ca2+ concentration. Coelentrazine-h exhibits an approximate 16-fold greater luminescence intensity (emission max.: ~466 nm; half-time total of 0.6-1.2 s) as compared to the native Coelenterazine (Cat. No. 233900). Has been used to measure intracellular Ca2+ signals in Dictyostelium discoideum chemotaxis and in plant wound healing.
FormYellow brown solid
Intert gas (Yes/No) Packaged under inert gas
CAS number50909-86-9
Chemical formulaC₂₆H₂₁N₃O₂
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityEthanol (10 mg/ml) or Methanol (10 mg/ml)
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution aliquot, purge with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesNebl, T., and Fisher. P.R. 1997. J. Cell. Sci. 110, 2845.
Shimomura, O. 1997. Biochem. J. 326, 297.
Sanchez-Bueno, A., et al. 1996. Int. J. Biochem. Cell Biol. 28, 1045.
Knight, M.R., et al. 1993. J. Cell. Biol. 121, 83.
Shimomura, O., et al. 1993. Biochem. J. 296, 549.
Shimomura, O., et al. 1993. Cell Calcium 14, 373.
Shimomura, O., et al. 1989. Biochem. J. 261, 913.